Human Aquaporin 3, AQP-3 ELISA Kit | AQP-3 elisa kit

Human Aquaporin 3, AQP-3 ELISA Kit

Cat. Num. AAA163805

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Human
• Synonyms: Aquaporin 3; AQP-3; Human Aquaporin 3; AQP-3 ELISA Kit; AQP-3 elisa kit

• Reactivity: Human
• Sequence Length: 292

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05-15ng/ml
• Sensitivity: 0.027ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-3 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Aquaporin 3 (also known as AQP-3) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human AQP-3 antibody. AQP-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AQP-3 Antibody is added and binds to AQP-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AQP-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AQP-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4826645
• NCBI GeneID: 360
• NCBI Accession #: NP_004916.1
• NCBI GenBank Nucleotide #: NM_004925.4
• UniProt Accession #: Q92482; O00108; Q6FGT2; Q6FGW6; A8K843; B2RE16; D3DRL3
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: aquaporin-3 isoform 1
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3
• UniProt Protein Name: Aquaporin-3
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism ().