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Typical Testing Data/Standard Curve (for reference only)

Canine Glycerol-3-phosphate acyltransferase (GPAT) ELISA Kit | GPAT elisa kit

Canine Glycerol-3-phosphate acyltransferase (GPAT) ELISA Kit

Reactivity
Canine
Synonyms
Glycerol-3-phosphate acyltransferase (GPAT); Canine Glycerol-3-phosphate acyltransferase (GPAT) ELISA Kit; GPAT elisa kit
Ordering
For Research Use Only!
Reactivity
Canine
Sequence Length
410
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 30ng/ml
Sensitivity
0.022ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GPAT elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Glycerol-3-phosphate Acyltransferase (also known as GPAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine GPAT antibody. GPAT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine GPAT Antibody is added and binds to GPAT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPAT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine GPAT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
46,007 Da
NCBI Official Full Name
GPAT
UniProt Protein Name
Glycerol-3-phosphate acyltransferase, chloroplastic
UniProt Gene Name
GPAT
UniProt Synonym Gene Names
GPAT

Uniprot Description

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids.

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Product Notes

The Canine GPAT gpat (Catalog #AAA1602621) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1602621 ELISA Kit recognizes Canine GPAT. It is sometimes possible for the material contained within the vial of "Glycerol-3-phosphate acyltransferase (GPAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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