Fish Aspartate aminotransferase (AST) ELISA Kit | AST elisa kit

Fish Aspartate aminotransferase (AST) ELISA Kit

Cat. Num. AAA1601734

• Gene Names: AAT; aspartate aminotransferase; ASPARTATE AMINOTRANSFERASE; ATAAT; MATERNAL EFFECT EMBRYO ARREST 17; MEE17; T26C19.9; T26C19_9
• Reactivity: Fish
• Synonyms: Aspartate aminotransferase (AST); Fish Aspartate aminotransferase (AST) ELISA Kit; AST elisa kit

• Reactivity: Fish
• Sequence Length: 475

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 60ng/ml
• Sensitivity: 0.09ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AST elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Fish Aspartate Aminotransferase (also known as AST) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Fish AST antibody. AST present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Fish AST Antibody is added and binds to AST in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AST antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Fish AST. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 79322722
• NCBI GeneID: 816758
• NCBI Accession #: NP_001031394.1
• NCBI GenBank Nucleotide #: NM_001036317.1
• UniProt Accession #: Q9SIE1; Q3E6N9; Q8LFX1; Q8VZI1; E9L7A7
• Molecular Weight: 46,039 Da
• NCBI Official Full Name: aspartate aminotransferase
• NCBI Official Symbol: AAT
• NCBI Official Synonym Symbols: aspartate aminotransferase; ASPARTATE AMINOTRANSFERASE; ATAAT; MATERNAL EFFECT EMBRYO ARREST 17; MEE17; T26C19.9; T26C19_9
• NCBI Protein Information: aspartate aminotransferase
• UniProt Protein Name: Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase
• Protein Family: Allatostatin
• UniProt Gene Name: PAT
• UniProt Synonym Gene Names: AAT; MEE17; AtAAT; AtPPA-AT

NCBI Description

Encodes a prokaryotic-type plastidic aspartate aminotransferase with glutamate/aspartate-prephenate aminotransferase (PAT) activity.

Uniprot Description

Prokaryotic-type aspartate aminotransferase. Has also a prenate transaminase activity. Involved in the aromatic amino acids biosynthesis pathway via the arogenate route. Required for the transamination of prephenate into arogenate. Required for early development of the embryo.