Typical Testing Data/Standard Curve (for reference only)
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Human beta defensin 2, HBD2 ELISA Kit | HBD2 elisa kit
Human beta defensin 2, HBD2 ELISA Kit
Gene Names
DEFB4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
Reactivity
Human
Synonyms
beta defensin 2; HBD2; Human beta defensin 2; HBD2 ELISA Kit; HBD2 elisa kit
Reactivity
Human
Sequence Length
64
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
10-4000ng/L
Sensitivity
5.31ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for HBD2 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human beta defensin 2 (also known as HBD2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human HBD2 antibody. HBD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HBD2 Antibody is added and binds to HBD2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HBD2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HBD2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human HBD2 antibody. HBD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HBD2 Antibody is added and binds to HBD2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HBD2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HBD2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,038 Da
NCBI Official Full Name
beta defensin 2
NCBI Official Synonym Full Names
defensin beta 4A
NCBI Official Symbol
DEFB4A
NCBI Official Synonym Symbols
BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
NCBI Protein Information
beta-defensin 4A
UniProt Protein Name
Beta-defensin 4A
UniProt Gene Name
DEFB4A
UniProt Synonym Gene Names
DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008]
Uniprot Description
Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed:20068036).
Research Articles on HBD2
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Product Notes
The Human HBD2 defb4a (Catalog #AAA160116) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA160116 ELISA Kit recognizes Human HBD2. It is sometimes possible for the material contained within the vial of "beta defensin 2, HBD2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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