Typical Testing Data/Standard Curve (for reference only)
Rat Transcription Factor A, Mitochondrial (TFAM) ELISA Kit | TFAM elisa kit
Rat Transcription Factor A, Mitochondrial (TFAM) ELISA Kit
Reactivity
Rat
Synonyms
Transcription Factor A; Mitochondrial (TFAM); Rat Transcription Factor A; Mitochondrial (TFAM) ELISA Kit; TFAM elisa kit
Reactivity
Rat
Sequence Length
149
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
7g/L - 1500ng/L
Sensitivity
3.52ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TFAM elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Transcription Factor A,Mitochondrial (also known as TFAM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TFAM antibody. TFAM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TFAM Antibody is added and binds to TFAM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TFAM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TFAM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TFAM antibody. TFAM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TFAM Antibody is added and binds to TFAM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TFAM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TFAM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,726 Da
NCBI Official Full Name
transcription factor A, partial
NCBI Official Symbol
TFAM
NCBI Protein Information
transcription factor A, mitochondrial
UniProt Protein Name
Transcription factor A, mitochondrial
Protein Family
UniProt Gene Name
TFAM
UniProt Synonym Gene Names
mtTFA
Uniprot Description
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA ().
Research Articles on TFAM
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Product Notes
The Rat TFAM tfam (Catalog #AAA1600609) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600609 ELISA Kit recognizes Rat TFAM. It is sometimes possible for the material contained within the vial of "Transcription Factor A, Mitochondrial (TFAM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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