TOR recombinant protein

Recombinant Mouse-ear cress TOR protein, N-His Tag

Cat. Num. AAA1562970

• Gene Names: TOR; F2J10.19; F2J10_19; target of rapamycin
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TOR; Recombinant Mouse-ear cress TOR protein; N-His Tag; Serinethreonine-protein kinase TOR AtTOR Protein TARGET OF RAPAMYCIN TOR; TOR recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: N-His Tag
• Species: Arabidopsis thaliana (Mouse-ear cress)
• Protein Construction: A DNA sequence encoding the Mouse-ear cress TOR (Met1113-Pro1304) was fused with the N-His Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder or frozen liquid. They are shipped out with dry ice or blue ice unless customers require otherwise.

Product Categories/Family for TOR recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 22330143
• NCBI GeneID: 841427
• NCBI Accession #: NP_175425.2
• NCBI GenBank Nucleotide #: NM_103891.4
• UniProt Accession #: Q9FR53; Q9LPM4
• Molecular Weight: 279,190 Da
• NCBI Official Full Name: target of rapamycin
• NCBI Official Symbol: TOR
• NCBI Official Synonym Symbols: F2J10.19; F2J10_19; target of rapamycin
• NCBI Protein Information: target of rapamycin
• UniProt Protein Name: Serine/threonine-protein kinase TOR
• Protein Family: Torsin
• UniProt Gene Name: TOR
• UniProt Synonym Gene Names: AtTOR

NCBI Description

Related to TOR proteins from yeast and mammals, regulators of cell growth in response to nutrient availability. TOR proteins belong to the family of phosphatidylinositol 3-kinase and are targets of the antiproliferative drug rapamycin. AtTOR binds the yeast FKBP12 protein in the presence of Rapamycin, is involved in embryogenesis and is expressed in embryos, endosperm and meristems.

Uniprot Description

Essential cell growth regulator that controls development from early embryo to seed production. Controls plant growth in environmental stress conditions. Acts through the phosphorylation of downstream effectors that are recruited by the binding partner RAPTOR. Acts by activating transcription, protein synthesis and ribosome biogenesis, and inhibiting mRNA degradation and autophagy. Can phosphorylate TAP46, a regulatory subunit of protein phosphatase 2A that modulates cell growth and survival (PubMed:21216945). Involved in modulating the transition from heterotrophic to photoautotrophic growth by regulating the expression of chloroplast- and photosynthesis-associated genes (PubMed:27479935). Essential for auxin signaling transduction, probably acting in polysomes to maintain the active ATPK1/S6K1 (and thus TIF3H1/eIF3h) phosphorylation status that is critical for translation reinitiation (e.g. uORF-mRNAs loading) (PubMed:23524850, PubMed:27014314). Promotes abscisic acid (ABA) biosynthesis (PubMed:26459592). Involved in the regulation of sugar-mediated (e.g. glucose and sucrose) glycolysis- and mitochondrial bioenergetics-dependent root growth promotion (PubMed:23542588). Required for sugar (e.g. glucose) promotion of hypocotyl elongation in the dark, by activating the brassinosteroid pathway and stabilizing BZR1. The regulation of BZR1 degradation is dependent on autophagy (PubMed:27345161).