EEF2 recombinant protein

Recombinant Human EEF2 protein

Cat. Num. AAA1562464

• Gene Names: EEF2; EF2; EF-2; EEF-2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EEF2; Recombinant Human EEF2 protein; Elongation factor 2; Short name:EF-2; EEF2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human EEF2 (Val509-Leu858) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for EEF2 recombinant protein

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Product Categories/Family for EEF2 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4503483
• NCBI GeneID: 1938
• NCBI Accession #: NP_001952.1
• NCBI GenBank Nucleotide #: NM_001961.3
• UniProt Accession #: P13639; Q58J86; B2RMP5; D6W618
• Molecular Weight: 95,338 Da
• NCBI Official Full Name: elongation factor 2
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 2
• NCBI Official Symbol: EEF2
• NCBI Official Synonym Symbols: EF2; EF-2; EEF-2
• NCBI Protein Information: elongation factor 2; polypeptidyl-tRNA translocase
• UniProt Protein Name: Elongation factor 2
• Protein Family: Elongation factor
• UniProt Gene Name: EEF2
• UniProt Synonym Gene Names: EF2; EF-2
• UniProt Entry Name: EF2_HUMAN

NCBI Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: polysome; membrane; cytoplasm; plasma membrane; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of translation; translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; pathogenesis; gene expression; hemopoietic progenitor cell differentiation; post-translational protein modification

Disease: Spinocerebellar Ataxia 26