EphA4 recombinant protein

Recombinant Human EphA4 protein, C-Fc Tag

Cat. Num. AAA1560760

• Gene Names: EPHA4; SEK; HEK8; TYRO1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EphA4; Recombinant Human EphA4 protein; C-Fc Tag; HEK8; SEK; TYRO1; EphA4 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: C-Fc Tag
• Species: Homo sapiens (Human)
• Endotoxin Level: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the human EPHA4 (Val20-Thr547) was fused with the C-terminal Fc Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Categories/Family for EphA4 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4758280
• NCBI GeneID: 2043
• NCBI Accession #: NP_004429.1
• NCBI GenBank Nucleotide #: NM_004438.3
• UniProt Accession #: P54764; Q2M380
• Molecular Weight: 109,860 Da
• NCBI Official Full Name: ephrin type-A receptor 4
• NCBI Official Synonym Full Names: EPH receptor A4
• NCBI Official Symbol: EPHA4
• NCBI Official Synonym Symbols: SEK; HEK8; TYRO1
• NCBI Protein Information: ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA4
• UniProt Synonym Gene Names: HEK8; SEK; TYRO1; EK8; hEK8
• UniProt Entry Name: EPHA4_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; axon; early endosome membrane; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium

Molecular Function: identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; motor axon guidance; regulation of astrocyte differentiation; positive regulation of dendrite morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior; ephrin receptor signaling pathway; corticospinal tract morphogenesis; cell adhesion