CD142 recombinant protein

Recombinant Human CD142 protein, C-His Tag

Cat. Num. AAA1560582

• Gene Names: F3; TF; TFA; CD142
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD142; Recombinant Human CD142 protein; C-His Tag; F3; CD142 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: C-His Tag
• Species: Homo sapiens (Human)
• Endotoxin Level: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the human F3 (Met1-Glu251) was fused with the C-terminal His Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

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Product Categories/Family for CD142 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 296010912
• NCBI GeneID: 2152
• NCBI Accession #: NP_001171567.1
• NCBI GenBank Nucleotide #: NM_001178096.1
• UniProt Accession #: P13726; Q6FHG2; Q86WH4; D3DT47
• Molecular Weight: 33,068 Da
• NCBI Official Full Name: tissue factor isoform 2
• NCBI Official Synonym Full Names: coagulation factor III (thromboplastin, tissue factor)
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: TF
• UniProt Entry Name: TF_HUMAN

NCBI Description

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: extracellular matrix; extracellular space; cell surface; integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protease binding; phospholipid binding

Biological Process: caspase activation; activation of blood coagulation via clotting cascade; positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; positive regulation of angiogenesis; activation of plasma proteins during acute inflammatory response; positive regulation of positive chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of cell migration