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Immunofluorescence (IF)

Rabbit CKM Polyclonal Antibody | anti-CKM antibody

Anti CKM polyclonal antibody

Gene Names
CKM; CKMM; M-CK
Reactivity
Human
Other species were not tested
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
CKM; Polyclonal Antibody; Anti CKM polyclonal antibody; Creatine kinase M-type; CPK-M; CK-MM; anti-CKM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Other species were not tested
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Form/Format
Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CKM antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:2000-1:8000
IHC: 1:200-1:400
Immunogen
Recombinant protein of human CKM (Met1-Lys381)
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 degree C for frequentUse. Store at -20 to -80 degree C for twelve months from the date of receipt.

Immunofluorescence (IF)

Immunofluorescence (IF)

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-CKM antibody
CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Product Categories/Family for anti-CKM antibody
References
PubMed: 3778496; PubMed: 15489334; PMID: 3034271

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,101 Da
NCBI Official Full Name
creatine kinase M-type
NCBI Official Synonym Full Names
creatine kinase, muscle
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
CKMM; M-CK
NCBI Protein Information
creatine kinase M-type; creatine kinase M chain
UniProt Protein Name
Creatine kinase M-type
Protein Family
UniProt Gene Name
CKM
UniProt Synonym Gene Names
CKMM
UniProt Entry Name
KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: EC 2.7.3.2; Kinase, other; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process

Research Articles on CKM

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Product Notes

The CKM ckm (Catalog #AAA1560201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti CKM polyclonal antibody reacts with Human Other species were not tested and may cross-react with other species as described in the data sheet. AAA Biotech's CKM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:2000-1:8000 IHC: 1:200-1:400. Researchers should empirically determine the suitability of the CKM ckm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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