Mouse surfactant protein B ELISA Kit | SFTPB elisa kit

Mouse Pulmonary Surfactant-associated protein B, SP-B ELISA Kit

Cat. Num. AAA15585

• Gene Names: Sftpb; SF-B; SP-B; Sftp3; Sftp-3; AI562151
• Reactivity: Mouse
• Synonyms: surfactant protein B; N/A; Mouse Pulmonary Surfactant-associated protein B, SP-B ELISA Kit; Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; SFTPB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SP-B. No significant cross-reactivity or interference between mouse SP-B and analogues was observed.
• Sequence Length: 377
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.45 ng/ml-30 ng/ml
• Sensitivity: < 0.11 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SFTPB elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 22296601
• NCBI GeneID: 20388
• NCBI Accession #: NP_680088.1
• NCBI GenBank Nucleotide #: NM_147779.2
• UniProt Accession #: P50405
• Molecular Weight: 41,728 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B isoform 1
• NCBI Official Synonym Full Names: surfactant associated protein B
• NCBI Official Symbol: Sftpb
• NCBI Official Synonym Symbols: SF-B; SP-B; Sftp3; Sftp-3; AI562151
• NCBI Protein Information: pulmonary surfactant-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe)
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• UniProt Gene Name: Sftpb
• UniProt Synonym Gene Names: Sftp3; SP-B
• UniProt Entry Name: PSPB_MOUSE