CTLA4 antibody set

CTLA4 Detection Set (Risk Free)

Cat. Num. AAA154697

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Antibodies are supplied as protein A purified IgG1
• Synonyms: CTLA4; Monoclonal Antibody Set; CTLA4 Detection Set (Risk Free); CTLA4 antibody set

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Antibodies are supplied as protein A purified IgG1
• Form/Format: Liquid. PBS containing 0.02% sodium azide.
• Concentration: Antibody 1mg/mL (varies by lot)

• Applicable Applications for CTLA4 antibody set: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Immunogen: Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.
• Conjugation: Unconjugated
• Preparation and Storage: Antibodies can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Related Product Information for CTLA4 antibody set

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treaent of cancer (5). Risk-Free antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-Free means they are guaranteed to work for their approved applications

References

Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4. Nature 1987; 328:267-70.
Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405-13.
Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607-9.
Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.