Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, MBS154639, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1ug/mL)
Mouse COVID 19 Nucleocapsid (NP) Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
ELISA, Immunofluorescence, Western Blot
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus; Monoclonal Antibody; SARS-CoV-2 (COVID-19) Nucleoprotein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); anti-COVID-19 antibody
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
Mouse IgG2b
Specificity
Specific for the nucleoprotein of the SARS virus.
Purity/Purification
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Form/Format
Liquid; SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Optimal dilutions for each application to be determined by the researcher.
Conjugation
Unconjugated
Preparation and Storage
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody can be stored at -20 degree C, stable for one year.
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, MBS154639, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1ug/mL)
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, MBS154639, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1ug/mL)
Testing Data
(Figure 2 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, MBS154639. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugateat 1:20000 dilution. Detection range is from 1 ng/mL to 2000 ng/mL.)
Testing Data
(Figure 2 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, MBS154639. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugateat 1:20000 dilution. Detection range is from 1 ng/mL to 2000 ng/mL.)
Product Categories/Family for anti-COVID-19 antibody
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Product Notes
The COVID-19 (Catalog #AAA154639) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Nucleoprotein reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Nucleocapsid (NP) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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