Rabbit anti-Human DFF45 Polyclonal Antibody | anti-DFFA antibody
DFF45 Antibody
Western Blot (WB)
(Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.)
Immunocytochemistry (IHC)
(Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 μg/mL.)
NCBI and Uniprot Product Information
NCBI Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase
Chromosomal Location of Human Ortholog: 1p36.3-p36.2
Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; cytosol; nucleus
Molecular Function: protein binding
Biological Process: apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis