Rabbit PRDM16 Polyclonal Antibody | anti-PRDM16 antibody

PRDM16 Antibody

Cat. Num. AAA150905

• Gene Names: PRDM16; MEL1; LVNC8; PFM13; CMD1LL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PRDM16 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PRDM16; Polyclonal Antibody; PRDM16 Antibody; MEL1; LVNC8; PFM13; CMD1LL; KIAA1675; PR domain zinc finger protein 16; PR domain-containing protein 16; PR domain containing 16; anti-PRDM16 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PRDM16 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1,092

• Applicable Applications for anti-PRDM16 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16.
• Buffer: PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PRDM16 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PRDM16 in human brain tissue with PRDM16 antibody at 2 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PRDM16 in mouse brain tissue with PRDM16 antibody at 20 μg/ml.Green: PRDM16 Antibody (5555)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of PRDM16 in mouse brain tissue with PRDM16 antibody at 2 μg/ml.)

Related Product Information for anti-PRDM16 antibody

PRDM16 Antibody: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.

NCBI and Uniprot Product Information

• NCBI GI #: 55665817
• NCBI GeneID: 63976
• UniProt Accession #: Q9HAZ2; Q8WYJ9; Q9C0I8; A6NHQ8; B1AJP7; B1AJP8; B1AJP9; B1WB48
• Molecular Weight: 120,338 Da
• NCBI Official Synonym Full Names: PR domain containing 16
• NCBI Official Symbol: PRDM16
• NCBI Official Synonym Symbols: MEL1; LVNC8; PFM13; CMD1LL
• NCBI Protein Information: PR domain zinc finger protein 16
• UniProt Protein Name: PR domain zinc finger protein 16
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM16
• UniProt Synonym Gene Names: KIAA1675; MEL1; PFM13; MDS1/EVI1-like gene 1
• UniProt Entry Name: PRD16_HUMAN

NCBI Description

The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM16: binds DNA and functions as a transcriptional regulator. A repressor of TGF-beta signaling. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. Four human isoforms are produced by alternative promoter usage and alternative splicing

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p36.23-p33

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: protein binding; transcription activator binding; sequence-specific DNA binding; metal ion binding; transcription coactivator activity; SMAD binding

Biological Process: regulation of cellular respiration; tongue development; neurogenesis; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; white fat cell differentiation; brown fat cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of granulocyte differentiation

Disease: Left Ventricular Noncompaction 8