Western Blot (WB)
(Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.)
Rabbit anti-Human CASKIN2 Polyclonal Antibody | anti-CASKIN2 antibody
CASKIN2 Antibody
Gene Names
CASKIN2; ANKS5B
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
CASKIN2 Antibody is affinity chromatography purified via peptide column.
Synonyms
CASKIN2; Polyclonal Antibody; CASKIN2 Antibody; ANKS5B; KIAA1139; Caskin-2; CASK-interacting protein 2; CASK interacting protein 2; anti-CASKIN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.
Purity/Purification
CASKIN2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1202
Applicable Applications for anti-CASKIN2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 5 mug/mL.
Conjugate
Unconjugated
Immunogen
CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2.
Buffer
CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
CASKIN2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.)
Related Product Information for anti-CASKIN2 antibody
CASKIN2 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
118,157 Da
NCBI Official Full Name
caskin-2 isoform a
NCBI Official Synonym Full Names
CASK interacting protein 2
NCBI Official Symbol
CASKIN2
NCBI Official Synonym Symbols
ANKS5B
NCBI Protein Information
caskin-2
UniProt Protein Name
Caskin-2
Protein Family
UniProt Gene Name
CASKIN2
UniProt Synonym Gene Names
KIAA1139
UniProt Entry Name
CSKI2_HUMAN
NCBI Description
This gene encodes a large protein that contains six ankyrin repeats, as well as a Src homology 3 (SH3) domain and two sterile alpha motif (SAM) domains, which may be involved in protein-protein interactions. The C-terminal portion of this protein is proline-rich and contains a conserved region. A related protein interacts with calcium/calmodulin-dependent serine protein kinase (CASK). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Uniprot Description
CASKIN2: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: cytoplasm
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Product Notes
The CASKIN2 caskin2 (Catalog #AAA150096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CASKIN2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASKIN2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 5 mug/mL. Researchers should empirically determine the suitability of the CASKIN2 caskin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASKIN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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