Rabbit anti-Mouse Dual specificity mitogen-activated protein kinase kinase 4 Polyclonal Antibody | anti-Mkk4 antibody

Rabbit anti-mouse Dual specificity mitogen-activated protein kinase kinase 4 polyclonal Antibody

Cat. Num. AAA1497245

• Gene Names: Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 4; Polyclonal Antibody; Rabbit anti-mouse Dual specificity mitogen-activated protein kinase kinase 4 polyclonal Antibody; C-JUN N-terminal kinase kinase 1; JNK-activating kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; Jnkk1; Mek4; Prkmk4; Sek1; Serk1; Skk1; Map2k4; anti-Mkk4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 397

• Applicable Applications for anti-Mkk4 antibody: ELISA (EIA), Western Blot (WB)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant mouse Dual specificity mitogen-activated protein kinase kinase 4 protein(2-397a)
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Raw264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue,Mouse brain tissueAll lanes: Map2k4 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 45 KDaObserved band size: 45 KDa)

Related Product Information for anti-Mkk4 antibody

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

NCBI and Uniprot Product Information

• NCBI GI #: 22095023
• NCBI GeneID: 26398
• NCBI Accession #: NP_033183.1
• NCBI GenBank Nucleotide #: NM_009157.5
• UniProt Accession #: P47809
• Molecular Weight: 44,114 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4 isoform b
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: Map2k4
• NCBI Official Synonym Symbols: MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k4
• UniProt Synonym Gene Names: Jnkk1; Mek4; Mkk4; Prkmk4; Sek1; Serk1; Skk1; MAP kinase kinase 4; MAPKK 4; JNK kinase 1; JNKK 1; MEK 4; SEK1
• UniProt Entry Name: MP2K4_MOUSE

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Protein kinase, STE; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; STE group; STE7 family

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; nucleus; perikaryon

Molecular Function: ATP binding; JUN kinase kinase activity; kinase activity; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: activation of JNK activity; activation of protein kinase activity; apoptosis; JNK cascade; MAPKKK cascade; phosphorylation; positive regulation of apoptosis; positive regulation of DNA replication; positive regulation of neuron apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle; response to wounding; stress-activated protein kinase signaling pathway