Western Blot (WB)
(Western blotAll lanes: DNA ligase antibody at 2ug/ml+DH5a whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 74 kDaObserved band size: 74 kDa)
Rabbit DNA Polyclonal Antibody | anti-DNA antibody
Rabbit anti-Escherichia coli DNA ligase polyclonal Antibody
Gene Names
ligA; dnaL; ECK2406; JW2403; lig; lop; pdeC
Reactivity
Escherichia coli
Applications
ELISA, Western Blot
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
DNA; Polyclonal Antibody; Rabbit anti-Escherichia coli DNA ligase polyclonal Antibody; Polydeoxyribonucleotide synthase [NAD(+)]; ligA; dnaL; lig; lop; pdeC; b2411; JW2403; anti-DNA antibody
Host
Rabbit
Reactivity
Escherichia coli
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
671
Applicable Applications for anti-DNA antibody
ELISA (EIA), Western Blot (WB)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant Escherichia coli DNA ligase protein
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western blotAll lanes: DNA ligase antibody at 2ug/ml+DH5a whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 74 kDaObserved band size: 74 kDa)
Western Blot (WB)
(Western blotAll lanes: DNA ligase antibody at 2ug/ml+DH5a whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 74 kDaObserved band size: 74 kDa)
Related Product Information for anti-DNA antibody
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,606 Da
NCBI Official Full Name
DNA ligase, NAD(+)-dependent
NCBI Official Symbol
ligA
NCBI Official Synonym Symbols
dnaL; ECK2406; JW2403; lig; lop; pdeC
NCBI Protein Information
DNA ligase, NAD(+)-dependent
UniProt Protein Name
DNA ligase
Protein Family
UniProt Gene Name
ligA
UniProt Synonym Gene Names
dnaL; lig; lop; pdeC
UniProt Entry Name
DNLJ_ECOLI
NCBI Description
ligA is an essential gene. [More information is available at EcoGene: EG10534]. LigA is one of two known NAD(+)-dependent DNA ligases, catalyzing the formation of phosphodiester bonds in duplex DNA. [More information is available at EcoCyc: EG10534].
Uniprot Description
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Research Articles on DNA
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Product Notes
The DNA liga (Catalog #AAA1488469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli DNA ligase polyclonal Antibody reacts with Escherichia coli and may cross-react with other species as described in the data sheet. AAA Biotech's DNA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the DNA liga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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