Actin, beta Human Active Protein | ACTB active protein
Actin, beta Human
Gene Names
ACTB; BRWS1; PS1TP5BP1
Applications
ELISA, Western Blot
Purity
>99%. May contain traces of gamma-actin.
Synonyms
Actin; beta Human; ACTB active protein
Host
Purified beta-Actin from Human platelets cells.
Purity/Purification
>99%. May contain traces of gamma-actin.
Form/Format
Supplied as a liquid in 5mM Tris. pH 8, 0.2mM calcium chloride, 0.2mM ATP, 5% sucrose, 1% dextran.
Concentration
1mg/ml (varies by lot)
Applicable Applications for ACTB active protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Recommended Dilution:
ELISA: 1ug/ml Used to coat plates
Western Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed.
Other applications not tested. Optimal dilution to be detemined by the researcher.
ELISA: 1ug/ml Used to coat plates
Western Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed.
Other applications not tested. Optimal dilution to be detemined by the researcher.
Biological Activity
Not tested. If the protein is stored and used properly can be used for biological assays.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeating freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Related Product Information for ACTB active protein
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (gsmooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western blots.
Product Categories/Family for ACTB active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~43kD
NCBI Official Full Name
Actin, beta
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
Similar Products
Product Notes
The ACTB actb (Catalog #AAA14745) is an Active Protein produced from Purified beta-Actin from Human platelets cells. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Actin, beta Human can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Recommended Dilution: ELISA: 1ug/ml Used to coat plates Western Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed. Other applications not tested. Optimal dilution to be detemined by the researcher. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, beta Human, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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