Phosphatase 1 Recombinant Protein | PPP1R3B recombinant protein

Recombinant Human Protein Phosphatase 1, Regulatory Subunit 3B

Cat. Num. AAA146465

• Gene Names: PPP1R3B; GL; PTG; PPP1R4
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Phosphatase 1; Recombinant Human Protein Phosphatase 1; Regulatory Subunit 3B; PPP1R3B Human; Protein Phosphatase 1; Regulatory Subunit 3B Human Recombinant; Protein phosphatase 1 regulatory subunit 3B; Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL; Protein phosphatase 1 regulatory subunit 4; PP1 subunit R4; Protein phosphatase 1 subunit GL; PTG; PPP1R4; PPP1R3B; GL; PPP1R3B recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The PPP1R3B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMAVDIE YRYNCMAPSL RQERFAFKIS PKPSKPLRPC IQLSSKNEAS GMVAPAVQEK KVKKRVSFAD NQGLALTMVK VFSEFDDPLD MPFNITELLD NIVSLTTAES ESFVLDFSQP SADYLDFRNR LQADHVCLEN CVLKDKAIAG TVKVQNLAFE KTVKIRMTFD TWKSYTDFPC QYVKDTYAGS DRDTFSFDIS LPEKIQSYER MEFAVYYECN GQTYWDSNRG KNYRIIRAEL KSTQGMTKPH SGPDLGISFD QFGSPRCSYG LFPEWPSYLG YEKLGPYY.
• Sequence Length: 285

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PPP1R3B recombinant protein

Description: PPP1R3B Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-285) and having a molecular mass of 35.1 kDa.PPP1R3B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Protein Phosphatase 1, Regulatory Subunit 3B (PPP1R3B) is the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. PPP1R3B, which is expressed both in liver and in skeletal muscle tissue, regulates glycogen synthesis in these tissues. PPP1R3B is involved in type 2 diabetes and maturity-onset diabetes of the young.

Product Categories/Family for PPP1R3B recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 318037218
• NCBI GeneID: 79660
• NCBI Accession #: NP_001188258.1
• NCBI GenBank Nucleotide #: NM_001201329.1
• UniProt Accession #: Q86XI6; Q9H812; B3KTV3
• Molecular Weight: 32,695 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 3B
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 3B
• NCBI Official Symbol: PPP1R3B
• NCBI Official Synonym Symbols: GL; PTG; PPP1R4
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 3B; PP1 subunit R4; hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL; hepatic glycogen-targeting subunit, G(L); protein phosphatase 1 regulatory subunit 4; protein phosphatase 1, regulatory (inhibitor) subunit 3B
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 3B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R3B
• UniProt Synonym Gene Names: PPP1R4; PP1 subunit R4; PTG
• UniProt Entry Name: PPR3B_HUMAN

NCBI Description

This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Jan 2011]

Uniprot Description

PPP1R3B: Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: intracellular membrane-bound organelle; glycogen granule; protein phosphatase type 1 complex

Molecular Function: enzyme binding; protein phosphatase regulator activity; phosphoprotein phosphatase activity

Biological Process: glycogen metabolic process; regulation of glycogen catabolic process; dephosphorylation; regulation of catalytic activity; regulation of glycogen biosynthetic process