Mesencephalic Astrocyte-Derived Neurotrophic Factor Active Protein | rMANF active protein
Recombinant Rat Mesencephalic Astrocyte-Derived Neurotrophic Factor
Gene Names
Manf; Armet
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Mesencephalic Astrocyte-Derived Neurotrophic Factor; Recombinant Rat Mesencephalic Astrocyte-Derived Neurotrophic Factor; MANF Rat; Mesencephalic Astrocyte-Derived Neurotrophic Factor Rat Recombinant; Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; Manf; Armet; rMANF active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Sequence Length
179
Solubility
It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 ug/ml, corresponding to a specific activity of >100 IU/mg.
Preparation and Storage
Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MANF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for rMANF active protein
Description: MANF Rat Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18.2kDa.
Introduction: MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
Introduction: MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
Product Categories/Family for rMANF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,388 Da
NCBI Official Full Name
mesencephalic astrocyte-derived neurotrophic factor isoform X1
NCBI Official Synonym Full Names
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Symbol
Manf
NCBI Official Synonym Symbols
Armet
NCBI Protein Information
mesencephalic astrocyte-derived neurotrophic factor; arginine-rich protein; arginine-rich, mutated in early stage tumors
UniProt Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
Protein Family
UniProt Gene Name
Manf
UniProt Synonym Gene Names
Armet
UniProt Entry Name
MANF_RAT
Uniprot Description
ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; perinuclear region of cytoplasm; endoplasmic reticulum; nucleus
Molecular Function: growth factor activity
Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein
Research Articles on rMANF
Similar Products
Product Notes
The rMANF manf (Catalog #AAA146286) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL. It is sometimes possible for the material contained within the vial of "Mesencephalic Astrocyte-Derived Neurotrophic Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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