WB (Western Blot)
(NCl6a-3 detects collagen XVII and lis 120kDa shed ectodomain by western blotting with keralJnocyte extract (E) and keratinocyte medium (M) respectively)
Mouse Collagen XVII Monoclonal Antibody | anti-COL17A1 antibody
Collagen XVII Mouse Monoclonal Antibody (NC16a-3)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Synonyms
Collagen XVII; Monoclonal Antibody; Collagen XVII Mouse Monoclonal Antibody (NC16a-3); Collagen alpha-1 (XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; BP180; BPAG2; anti-COL17A1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NC16a-3
Specificity
The antibody recognizes the NC16a domain (aa545-557)
Form/Format
Purified with protein G, stored in PBS pH 7.4 and lyophilized.
Applicable Applications for anti-COL17A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
If reconstituted with deionized water in 250 uL: WB 1:1000; IHC-P: 1:40. Optimal dilution has to be determined by the user
Preservative
None
Target
Collagen XVII
Target Alias
Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, COL17A1, BP180, BPAG2
Immunogen
Purified recombinant NC16a-domain of collagen XVII
Preparation and Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
WB (Western Blot)
(NCl6a-3 detects collagen XVII and lis 120kDa shed ectodomain by western blotting with keralJnocyte extract (E) and keratinocyte medium (M) respectively)
WB (Western Blot)
(NCl6a-3 detects collagen XVII and lis 120kDa shed ectodomain by western blotting with keralJnocyte extract (E) and keratinocyte medium (M) respectively)
Application Data
(Cryosections of normal human skin and from patients with inherited skin-blistering disorders and absence of collagen XVII or laminin 5 were stained with mAb NC16a-3 and a rabbit antHamlnin 5 antibody)
Application Data
(Cryosections of normal human skin and from patients with inherited skin-blistering disorders and absence of collagen XVII or laminin 5 were stained with mAb NC16a-3 and a rabbit antHamlnin 5 antibody)
Related Product Information for anti-COL17A1 antibody
Collagen XVII is a member of the transmembrane collagen family of proteins. It is a major structural component of the hemidesmosome (HD) playing an important role in its integrity. HD is a specialized multiprotein complex involved in the attachment of basal keratinocytes to the underlying basement membrane.
References
1. Hofmann SC-Plasmin plays a role in the in vitro generation of the linear IgA dermatosis antigen LADB97. 2. Seppanen A-Distribution of collagen XVII in the human brain. 3. Seppanen A-Expression of collagen XVII and ubiquitin-binding protein p62 in motor neuron disease.
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Product Notes
The COL17A1 (Catalog #AAA14613) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Collagen XVII can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). If reconstituted with deionized water in 250 uL: WB 1:1000; IHC-P: 1:40. Optimal dilution has to be determined by the user. Researchers should empirically determine the suitability of the COL17A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XVII, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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