Acireductone Dioxygenase 1 Recombinant Protein | ADI1 recombinant protein

Recombinant Human Acireductone Dioxygenase 1

Cat. Num. AAA145634

• Gene Names: ADI1; ARD; APL1; SIPL; mtnD; Fe-ARD; MTCBP1; Ni-ARD; HMFT1638
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Acireductone Dioxygenase 1; Recombinant Human Acireductone Dioxygenase 1; ADI1 Human; Acireductone Dioxygenase 1 Human Recombinant; APL1; ARD; FLJ10913; HMFT1638; MTCBP-1; SIPL; 1; 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Acireductone dioxygenase; Fe-ARD; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; Submergence-induced protein-like factor; ADI1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The ADI1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA
• Sequence Length: 179

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for ADI1 recombinant protein

Description: ADI1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 216 amino acids (1-179 a.a.) and having a molecular mass of 25.6kDa. ADI1 is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Acireductone dioxygenase 1 (ADI1) is a part of the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. ADI1 regulates mRNA processing in the nucleus, and carries out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. ADI1 down-regulates cell migration arbitrated by MMP14.

Product Categories/Family for ADI1 recombinant protein

Enzymes; Oxygenase

NCBI and Uniprot Product Information

• NCBI GI #: 171460928
• NCBI GeneID: 55256
• NCBI Accession #: NP_060739.2
• NCBI GenBank Nucleotide #: NM_018269.3
• UniProt Accession #: Q9BV57; Q53HW3; Q53QD3; Q57YV7; Q68CK2; Q6ZSF7; Q7Z512; Q96P85; Q9NV57; D6W4Y3
• Molecular Weight: 20,332 Da
• NCBI Official Full Name: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
• NCBI Official Synonym Full Names: acireductone dioxygenase 1
• NCBI Official Symbol: ADI1
• NCBI Official Synonym Symbols: ARD; APL1; SIPL; mtnD; Fe-ARD; MTCBP1; Ni-ARD; HMFT1638
• NCBI Protein Information: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; ADI1; ARD; MT1-MMP cytoplasmic tail-binding protein-1; MTCBP-1; acireductone dioxygenase (Fe(2+)-requiring); acireductone dioxygenase (Ni(2+)-requiring); fe-ARD; membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1; submergence induced protein 2; submergence-induced protein-like factor
• UniProt Protein Name: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
• Protein Family: Acireductone dioxygenase
• UniProt Gene Name: ADI1
• UniProt Entry Name: MTND_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. [provided by RefSeq, May 2010]

Uniprot Description

ADI1: Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. Belongs to the acireductone dioxygenase (ARD) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.13.11.54; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: acireductone dioxygenase [iron(II)-requiring] activity; protein binding; iron ion binding; oxidoreductase activity

Biological Process: methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process