Ubiquitin-Like Modifier Activating Enzyme 5 Recombinant Protein | UBA5 recombinant protein

Recombinant Human Ubiquitin-Like Modifier Activating Enzyme 5

Cat. Num. AAA145359

• Gene Names: UBA5; THIFP1; UBE1DC1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Ubiquitin-Like Modifier Activating Enzyme 5; Recombinant Human Ubiquitin-Like Modifier Activating Enzyme 5; UBA5 Human; Ubiquitin-Like Modifier Activating Enzyme 5 Human Recombinant; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; UBA5; UBE1DC1; UBA5 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The UBA5 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 50mM NaCl.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVENFQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM.
• Sequence Length: 404

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for UBA5 recombinant protein

Description: UBA5 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 428 amino acids (1-404) and having a molecular mass of 47.4kDa.UBA5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Ubiquitin-like modifier activating enzyme 5 (UBA5) is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates an ubiquitin-like protein, ubiquitin-fold modifier 1 (Ufm1), by forming a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.

Product Categories/Family for UBA5 recombinant protein

Enzymes; Ubiquitin Conjugating Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 13376212
• NCBI GeneID: 79876
• NCBI Accession #: NP_079094.1
• NCBI GenBank Nucleotide #: NM_024818.3
• UniProt Accession #: Q9GZZ9; Q96ST1; A6NJL3; D3DNC8
• Molecular Weight: 38,537 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; UFM1-activating enzyme; ubiquitin-activating enzyme 5; ubiquitin-activating enzyme E1 domain-containing protein 1; ubiquitin-activating enzyme E1-domain containing 1
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway