DNA topoisomerase 2-binding protein 1-A (topbp1-A) Recombinant Protein | topbp1-A recombinant protein

Recombinant Xenopus laevis DNA topoisomerase 2-binding protein 1-A (topbp1-A), partial

Cat. Num. AAA1452983

• Gene Names: topbp1.L; cut5; XtopBP; mus101; topbp1; Xmus101; top2bp1; TopBP1-A; TopBP1-B
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA topoisomerase 2-binding protein 1-A (topbp1-A); Recombinant Xenopus laevis DNA topoisomerase 2-binding protein 1-A (topbp1-A); partial; topbp1-A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,513

• Species: Xenopus laevis (African clawed frog)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 147904142
• NCBI GeneID: 398573
• NCBI Accession #: NP_001082568.1
• NCBI GenBank Nucleotide #: NM_001089099.1
• UniProt Accession #: Q800K6; B7ZSK6; Q7ZZY3
• Molecular Weight: 169,288 Da
• NCBI Official Full Name: DNA topoisomerase II binding protein 1 L homeolog
• NCBI Official Synonym Full Names: DNA topoisomerase II binding protein 1 L homeolog
• NCBI Official Symbol: topbp1.L
• NCBI Official Synonym Symbols: cut5; XtopBP; mus101; topbp1; Xmus101; top2bp1; TopBP1-A; TopBP1-B
• NCBI Protein Information: DNA topoisomerase II binding protein 1 L homeolog; topoisomerase (DNA) II binding protein 1 L homeolog
• UniProt Protein Name: DNA topoisomerase 2-binding protein 1-A
• Protein Family: DNA topoisomerase 2-binding protein
• UniProt Gene Name: topbp1-A
• UniProt Synonym Gene Names: TopBP1-A; XtopBP

Uniprot Description

Has dual roles in initiation of DNA replication, and regulation of checkpoint responses. Required for DNA replication initiation but not for the formation of pre-replicative complexes or the elongation stages. Necessary for the loading of replication factors onto chromatin, including gemc1, cdc45, DNA polymerases and components of the GINS complex such as ginsl/sld5. Binds chromatin in both S-phase cyclin-dependent kinase (S-CDK)-independent and S-CDK-dependent modes. Chromatin binding is required for the action of S-CDK, which in turn triggers the formation of preinitiation complexes of DNA replication. Its role in checkpoint activation is independent of the DNA replication role. In response to DNA damage, triggers the recruitment of checkpoint signaling proteins on chromatin, which activate the chek1 signaling pathway and block S-phase progression. Increases the kinase activity of atr to numerous substrates, and is required for the phosphorylation of Rad1.