DLAT/DLST/BCOADC Recombinant Protein | M2 recombinant protein
Recombinant Human DLAT/DLST/BCOADC
Applications
Western Blot, ELISA
Purity
Greater than 75% as determined by SDS-PAGE.
Synonyms
DLAT/DLST/BCOADC; Recombinant Human DLAT/DLST/BCOADC; M2 Human; DLAT/DLST/BCOADC Recombinant Human; M2 recombinant protein
Host
Sf9 Insect Cells
Purity/Purification
Greater than 75% as determined by SDS-PAGE.
Form/Format
M2 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Concentration
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (varies by lot)
Applicable Applications for M2 recombinant protein
Western Blot (WB), ELISA (EIA), Immunodot
Application Notes
Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody.
Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Immunological Functions
Binds IgG-type human auto-antibodies
Related Product Information for M2 recombinant protein
Introduction: M2 autoantigen is a key mark of antimitochondrial autoantibodies (AMA), a typical serological feature in patients suffering from primary biliary cirrhosis (PBC) which is a serious autoimmune liver disease accompanied by damage to intrahepatic bile ducts. Molecular definition of the M2 antigen has displayed it as no less than 3 separate target proteins. The M2 role is like the so-called E2 subunits (or dihydrolipoamide transferases) of different mitochondrial dehydrogenase complexes: *pyruvate dehydrogenase complex. *branched chain 2-oxo-acid dehydrogenase complex. *2-oxoglutarate dehydrogenase complex. Biochemically, these complexes catalyze the oxidative decarboxylation of various alpha-keto-acid substrates and systematically engage with a prosthetic lipoamide group; they are situated in the mitochondrial matrix in association with the inner membrane. The most well-known reactivity of AMA positive PBC sera is against PDC-E2. Some patients have AMA which reacts with PDC-E2 alone (95%), but most patients also show reactivity against OGDC-E2 (39-88%) and/or BCOADC-E2 (53-55%). Actually, patients can be found with reactivity only against OGDC-E2 and/or BCOADC-E2 and no PDC-E2 autoantibodies. These patients will be overlooked in assays based on natural source-derived, predominantly PDC-E2-containing M2 antigen preparations.
Product Categories/Family for M2 recombinant protein
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Product Notes
The M2 (Catalog #AAA145155) is a Recombinant Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DLAT/DLST/BCOADC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunodot. Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody. Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. Researchers should empirically determine the suitability of the M2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLAT/DLST/BCOADC, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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