Phosphatase 1 Recombinant Protein | PPP1R8 recombinant protein

Recombinant Human Protein Phosphatase 1, Regulatory Subunit 8

Cat. Num. AAA144704

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Phosphatase 1; Recombinant Human Protein Phosphatase 1; Regulatory Subunit 8; PPP1R8 Human; Protein Phosphatase 1; Regulatory Subunit 8 Human Recombinant; Protein phosphatase 1 regulatory subunit 8; nuclear inhibitor of protein phosphatase-1 alpha; Protein phosphatase 1 regulatory inhibitor subunit 8; ARD1; NIPP1; PRO2047; ARD-1; activator of RNA decay; nuclear subunit of PP-1; RNase E; PPP1R8 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The PPP1R8 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLGLEESG SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL IVEHHHHHH
• Sequence Length: 127

Related Product Information for PPP1R8 recombinant protein

Description: PPP1R8 Human Recombinant produced in E Coli is a single polypeptide chain containing 359 amino acids (1-351) and having a molecular mass of 39.5 kDa.PPP1R8 is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Introduction: Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) is the RNA-binding subunit of a key species of protein phosphatase-1 in the nucleus. The PPP1R8 enzyme is an inhibitor subunit of the main nuclear protein phosphatase-1 (PP-1). PPP1R8 has RNA-binding activity however it doesn't cleave RNA and may target PP-1 to RNA-associated substrates. Two isoforms of the PPP1R8 protein are specific inhibitors of type 1 serine/threonine protein phosphatases which can bind but not cleave RNA. The third isoform of the PPP1R8 protein is deficient in of the phosphatase inhibitory activity nonetheless it is a single-strand endoribonuclease comparable to RNase E of E. coli.

Product Categories/Family for PPP1R8 recombinant protein

Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI GeneID: 5511
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NM_002713.3
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 13,344 Da
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1; RNase E; activator of RNA decay; nuclear inhibitor of protein phosphatase-1 alpha; nuclear subunit of PP-1; protein phosphatase 1 regulatory subunit 8; protein phosphatase 1, regulatory (inhibitor) subunit 8
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit; EC 3.1.4.-; Ribonuclease; Motility/polarity/chemotaxis; Inhibitor

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; ribonuclease E activity; DNA binding; protein phosphatase type 1 regulator activity; RNA binding; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; negative regulation of catalytic activity; RNA catabolic process; mRNA processing