Wheat Gliadin Recombinant Protein
Recombinant Wheat Gliadin
Applications
Western Blot, ELISA
Purity
Greater than 94% as determined by SDS-PAGE.
Synonyms
Wheat Gliadin; Recombinant Wheat Gliadin; Gliadin Wheat; Gliadin Wheat Recombinant; Gliadin; Wheat Gliadin recombinant protein
Host
E Coli
Purity/Purification
Greater than 94% as determined by SDS-PAGE.
Form/Format
Gliadin (0.81mg/1ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl, and 20% glycerol.
Concentration
0.15-0.5 ug/ml (depending on the type of ELISA plate and coating buffer). (varies by lot)
Sequence Length
296
Applicable Applications for Wheat Gliadin recombinant protein
Western Blot (WB), ELISA (EIA), Immunodot
Application Notes
Western blot with monoclonal anti-hexa-His-tag antibody.
Standard ELISA test (checkerboard analysis of positive/negative sera panels)
Standard ELISA test (checkerboard analysis of positive/negative sera panels)
Appearance
Sterile Filtered clear solution
Immunological Functions
Binds IgG- and IgA-type human autoantibodies associated with celiac disease.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Related Product Information for Wheat Gliadin recombinant protein
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
Product Categories/Family for Wheat Gliadin recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
gliadin
UniProt Protein Name
Alpha/beta-type gliadin
UniProt Entry Name
Q41632_TRIUA
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Product Notes
The Wheat Gliadin (Catalog #AAA144477) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Wheat Gliadin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunodot. Western blot with monoclonal anti-hexa-His-tag antibody. Standard ELISA test (checkerboard analysis of positive/negative sera panels). Researchers should empirically determine the suitability of the Wheat Gliadin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wheat Gliadin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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