SDS-PAGE
Alpha-Fetoprotein Recombinant Protein | aFP recombinant protein
Recombinant Alpha-Fetoprotein (aFP)
Gene Names
AFP; AFPD; FETA; HPAFP
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
>95%
Synonyms
Alpha-Fetoprotein; N/A; Recombinant Alpha-Fetoprotein (aFP); aFP recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
NFSDSRT MHKNAYGIDS ILDSSPCSSG TNLVGLATIF FAQSVQGATY EEVSQMVKDV LTIIEKPTGS KQPAGCLENQ VSAFLEEICR EKEIPEKYGL SDCCSRTGEE RHDCFLAHKK AAPASIPPFP VLEPVTSCKS YKENRELFIN RYIYEIARRH PVLYAPTILS VANQYNKIIPHCCKAENATE CFETKVTSIT
NFSDSRT MHKNAYGIDS ILDSSPCSSG TNLVGLATIF FAQSVQGATY EEVSQMVKDV LTIIEKPTGS KQPAGCLENQ VSAFLEEICR EKEIPEKYGL SDCCSRTGEE RHDCFLAHKK AAPASIPPFP VLEPVTSCKS YKENRELFIN RYIYEIARRH PVLYAPTILS VANQYNKIIPHCCKAENATE CFETKVTSIT
Sequence Length
609
Applicable Applications for aFP recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Predicted Molecular Mass
25.6kDa
Accurate Molecular Mass (KD)
26kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag
Organism Species
Bos taurus; Bovine (Cattle)
Fragment
Asn14~Thr210
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,678 Da
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The aFP afp (Catalog #AAA144076) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha-Fetoprotein can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the aFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.NFSDSRT MHKNAYGIDS ILDSSPCSSG TNLVGLATIF FAQSVQGATY EEVSQMVKDV LTIIEKPTGS KQPAGCLENQ VSAFLEEICR EKEIPEKYGL SDCCSRTGEE RHDCFLAHKK AAPASIPPFP VLEPVTSCKS YKENRELFIN RYIYEIARRH PVLYAPTILS VANQYNKIIP HCCKAENATE CFETKVTSIT. It is sometimes possible for the material contained within the vial of "Alpha-Fetoprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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