Acid Phosphatase-1 Active Protein | ACP1 active protein

Recombinant Human Acid Phosphatase-1

Cat. Num. AAA143871

• Gene Names: ACP1; HAAP
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Acid Phosphatase-1; Recombinant Human Acid Phosphatase-1; ACP1 Human; Acid Phosphatase-1 Human Recombinant; HAAP; MGC3499; MGC111030; ACP1; Low molecular weight phosphotyrosine protein phosphatase; LMW-PTPase; LMW-PTP; Low molecular weight cytosolic acid phosphatase; Red cell acid phosphatase 1; Adipocyte acid phosphatase; ACP1 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The ACP1 protein solution contains 20mM MES, pH-6, 0.1mM PMSF, 2mM EDTA and 10% Glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIHTAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
• Sequence Length: 112

• Unit Defenition: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37 degree C in MES pH5.0 using 10mM of substrate.
• Specific Activity: 60,000 Units per 1mg.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for ACP1 active protein

Description: Recombinant Human ACP1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (1-158 a.a.) and having a molecular mass of 20.1 kDa. ACP1 is fused to a 20 amino acid His-Tag at N-Terminus and purified by conventional chromatography techniques.

Introduction: ACP1 is part of the phosphotyrosine protein. ACP1 functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) existing in all human tissues, including adipocytes. ACP1 enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. ACP1 is present in adipocytes, thus playing a specific role in the regulation of adipose tissue. High levels of the ACP1 negatively regulate cell proliferation and growth of leiomyomas during dephosphorylation of the PDGF receptor. High significant differences in birth weight-placental weight relationships were observed among acid phosphatase locus 1 phenotypes.

Product Categories/Family for ACP1 active protein

Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 96304457
• NCBI GeneID: 52
• NCBI Accession #: NP_001035739.1
• NCBI GenBank Nucleotide #: NM_001040649.2
• UniProt Accession #: P24666; P24667; Q16035; Q16036; Q16725; Q3KQX8; Q53RU0; A8K1L9; B5MCC7
• Molecular Weight: 12,230 Da
• NCBI Official Full Name: low molecular weight phosphotyrosine protein phosphatase isoform d
• NCBI Official Synonym Full Names: acid phosphatase 1, soluble
• NCBI Official Symbol: ACP1
• NCBI Official Synonym Symbols: HAAP
• NCBI Protein Information: low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; acid phosphatase of erythrocyte; adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; low molecular weight cytosolic acid phosphatase; protein tyrosine phosphatase; red cell acid phosphatase 1
• UniProt Protein Name: Low molecular weight phosphotyrosine protein phosphatase
• Protein Family: Adult cuticle protein
• UniProt Gene Name: ACP1
• UniProt Synonym Gene Names: LMW-PTP; LMW-PTPase
• UniProt Entry Name: PPAC_HUMAN

NCBI Description

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis; Phosphatase (non-protein); Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.2; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: internal side of plasma membrane; cytoplasm

Molecular Function: acid phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity