Heat Shock Protein 27 Recombinant Protein | HSP27 recombinant protein

Recombinant Human Heat Shock Protein 27, His Tag

Cat. Num. AAA143745

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Heat Shock Protein 27; Recombinant Human Heat Shock Protein 27; His Tag; HSP27 Human; His; Heat Shock Protein 27 Human Recombinant; HSPB1; CMT2F; HMN2B; HSP27; HSP28; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein; Stress-responsive protein 27; SRP27; HS.76067; DKFZp586P1322; HSP27 His; HSP27 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The HSP27 protein solution contains 20mM Tris-Hcl buffer, 1mM DTT and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
• Sequence Length: 205

• Preparation and Storage: HSP-27 his tag although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for HSP27 recombinant protein

Description: Recombinant Human HSP-27 produced in E Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 24.9kDa. Hsp27 is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography.

Introduction: HSP27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).

Product Categories/Family for HSP27 recombinant protein

Heat Shock Proteins; Recombinant Proteins; Heat Shock Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4504517
• NCBI GeneID: 3315
• NCBI Accession #: NP_001531.1
• NCBI GenBank Nucleotide #: NM_001540.3
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock 27kDa protein 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1; 28 kDa heat shock protein; HSP 27; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; cytosol; nucleus

Molecular Function: protein kinase C inhibitor activity; identical protein binding; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; retinal homeostasis; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; response to virus; positive regulation of blood vessel endothelial cell migration; negative regulation of protein kinase activity; gene expression; regulation of I-kappaB kinase/NF-kappaB cascade; cell motility; vascular endothelial growth factor receptor signaling pathway; response to unfolded protein; negative regulation of apoptosis; regulation of translational initiation

Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f