Thymus Expressed Chemokine Active Protein | TECK active protein

Recombinant Human Thymus Expressed Chemokine (CCL25)

Cat. Num. AAA143721

• Gene Names: CCL25; TECK; Ckb15; SCYA25
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Thymus Expressed Chemokine; Recombinant Human Thymus Expressed Chemokine (CCL25); TECK Human; Thymus Expressed Chemokine Human Recombinant (CCL25); C-C motif chemokine 25; Small-inducible cytokine A25; Thymus-expressed chemokine; Chemokine TECK; CCL25; SCYA25; TECK; Ckb15; MGC150327; TECK active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL
• Sequence Length: 149

• Solubility: It is recommended to reconstitute the lyophilized TECK in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.
• Preparation and Storage: Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TECK should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for TECK active protein

Description: TECK Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa. The TECK is purified by proprietary chromatographic techniques.

Introduction: CCL25 (Teck) is a novel CC chemokine, which is distantly related (about 20% amino acid sequence identity) to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development. Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.

Product Categories/Family for TECK active protein

Chemokines

NCBI and Uniprot Product Information

• NCBI GI #: 323098324
• NCBI GeneID: 6370
• NCBI Accession #: NP_001188288.1
• NCBI GenBank Nucleotide #: NM_001201359.1
• UniProt Accession #: O15444; Q96KJ7; A1L4J4; A6NI52; A8K9E7; B5MCA5
• Molecular Weight: 16,538 Da
• NCBI Official Full Name: C-C motif chemokine 25 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 25
• NCBI Official Symbol: CCL25
• NCBI Official Synonym Symbols: TECK; Ckb15; SCYA25
• NCBI Protein Information: C-C motif chemokine 25; Ck beta-15; TECKvar; chemokine TECK; small inducible cytokine subfamily A (Cys-Cys), member 25; small-inducible cytokine A25; thymus expressed chemokine; thymus-expressed chemokine
• UniProt Protein Name: C-C motif chemokine 25
• UniProt Gene Name: CCL25
• UniProt Synonym Gene Names: SCYA25; TECK
• UniProt Entry Name: CCL25_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine receptor binding; chemokine activity; CCR10 chemokine receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; immune response; positive regulation of cell-matrix adhesion; inflammatory response; chemotaxis; leukocyte migration