Glutaredoxin 5 Recombinant Protein | GLRX5 recombinant protein

Recombinant Human Glutaredoxin 5

Cat. Num. AAA143716

• Gene Names: GLRX5; GRX5; PRSA; FLB4739; PR01238; PRO1238; C14orf87
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Glutaredoxin 5; Recombinant Human Glutaredoxin 5; GLRX5 Human; Glutaredoxin 5 Human Recombinant; Glutaredoxin-related protein 5 mitochondrial; Monothiol glutaredoxin-5; GLRX5; C14orf87; glutaredoxin 5; GRX5; FLB4739; PR01238; PRO1238; MGC14129; GLRX5 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: GLRX5 solution containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.1M NaCl.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK
• Sequence Length: 157

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for GLRX5 recombinant protein

Description: GLRX5 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids (1-157 a.a.) and having a molecular mass of 18.8 kDa. GRX5 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: GLRX5 is small redox enzyme of approximately 100 amino acids which uses glutathione as a cofactor. GLRX5 is a mitochondrial protein, which is evolutionarily conserved. GLRX5 is oxidized by substrates, and reduced non-enzymatically by glutathione. GLRX5 is involved in the biogenesis of iron-sulfur clusters that are required for normal iron homeostasis. GLRX5 is necessary for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in the GLRX5 gene are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA).

Product Categories/Family for GLRX5 recombinant protein

Enzymes; Gluteradoxin

NCBI and Uniprot Product Information

• NCBI GI #: 42516576
• NCBI GeneID: 51218
• NCBI Accession #: NP_057501.2
• NCBI GenBank Nucleotide #: NM_016417.2
• UniProt Accession #: Q86SX6; Q0X088; Q3YML0; Q86WY3; Q8IZ54
• Molecular Weight: 16,628 Da
• NCBI Official Full Name: glutaredoxin-related protein 5, mitochondrial
• NCBI Official Synonym Full Names: glutaredoxin 5
• NCBI Official Symbol: GLRX5
• NCBI Official Synonym Symbols: GRX5; PRSA; FLB4739; PR01238; PRO1238; C14orf87
• NCBI Protein Information: glutaredoxin-related protein 5, mitochondrial; glutaredoxin 5 homolog; monothiol glutaredoxin-5
• UniProt Protein Name: Glutaredoxin-related protein 5, mitochondrial
• Protein Family: Glutaredoxin-related protein
• UniProt Gene Name: GLRX5
• UniProt Synonym Gene Names: C14orf87
• UniProt Entry Name: GLRX5_HUMAN

NCBI Description

This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010]

Uniprot Description

GLRX5: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA). A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus. Belongs to the glutaredoxin family. Monothiol subfamily.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: mitochondrion; nucleus

Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis; hemopoiesis

Disease: Anemia, Sideroblastic, Pyridoxine-refractory, Autosomal Recessive