Cytokeratin 14 Recombinant Protein | KRT14 recombinant protein

Recombinant Human Cytokeratin 14

Cat. Num. AAA142903

• Gene Names: KRT14; K14; NFJ; CK14; EBS3; EBS4
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Cytokeratin 14; Recombinant Human Cytokeratin 14; KRT14 Human; Cytokeratin 14 Human Recombinant; Keratin; type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT14; NFJ; CK14; EBS3; EBS4; KRT14 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 472

• Solubility: It is recommended to reconstitute the lyophilized CK-14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Reconstitution: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Preparation and Storage: Lyophilized KRT14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution KRT14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for KRT14 recombinant protein

Description: Cytokeratin 14 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton. The KRT14 is purified by proprietary chromatographic techniques.

Introduction: Cytokeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Product Categories/Family for KRT14 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 15431310
• NCBI GeneID: 3861
• NCBI Accession #: NP_000517.2
• NCBI GenBank Nucleotide #: NM_000526.4
• UniProt Accession #: P02533; Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
• Molecular Weight: 51,561 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 14
• NCBI Official Synonym Full Names: keratin 14, type I
• NCBI Official Symbol: KRT14
• NCBI Official Synonym Symbols: K14; NFJ; CK14; EBS3; EBS4
• NCBI Protein Information: keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• UniProt Protein Name: Keratin, type I cytoskeletal 14
• Protein Family: Keratin
• UniProt Gene Name: KRT14
• UniProt Synonym Gene Names: CK-14; K14
• UniProt Entry Name: K1C14_HUMAN

NCBI Description

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Uniprot Description

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: keratin filament; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development; epithelial cell differentiation; response to zinc ion; intermediate filament bundle assembly; response to ionizing radiation; hair cycle; aging

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type