Casein Kinase 2 alpha 1 Recombinant Protein | CSNK2A1 recombinant protein

Recombinant Human Casein Kinase 2 alpha 1

Cat. Num. AAA142754

• Gene Names: CSNK2A1; CKII; CK2A1; CSNK2A3
• Purity: Greater than 99% as determined by SDS-PAGE.
• Synonyms: Casein Kinase 2 alpha 1; Recombinant Human Casein Kinase 2 alpha 1; CSNK2A1 Human; Casein Kinase 2 alpha 1 Human Recombinant; Casein kinase II subunit alpha; EC 2.7.11.1; CK II; CK2A1; CKII alpha; CSNK2A1; PKCK2; CSK21; CSNK2A1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 99% as determined by SDS-PAGE.
• Form/Format: CSNK2A1 is supplied in 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500um PMSF, 50% glycerol, pH 8.5.; Sterile Filtered clear solution.
• Sequence Length: 391

• Unit Defenition: No protease activity detectable, specific activity > 1.320 U/mg (1U = 1 umol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Related Product Information for CSNK2A1 recombinant protein

Description: Casein Kinase 2 alpha 1 Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 45.1 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques.

Introduction: The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.

Product Categories/Family for CSNK2A1 recombinant protein

Protein Kinases; Enzymes; Casein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4503095
• NCBI GeneID: 1457
• NCBI Accession #: NP_001886.1
• NCBI GenBank Nucleotide #: NM_001895.3
• UniProt Accession #: P68400; P19138; P20426; Q14013; Q5U065; B4DYS6; D3DVV8; Q8NEV1
• Molecular Weight: 29,181 Da
• NCBI Official Full Name: casein kinase II subunit alpha isoform a
• NCBI Official Synonym Full Names: casein kinase 2, alpha 1 polypeptide
• NCBI Official Symbol: CSNK2A1
• NCBI Official Synonym Symbols: CKII; CK2A1; CSNK2A3
• NCBI Protein Information: casein kinase II subunit alpha; CK II alpha 3; CK2 catalytic subunit alpha; casein kinase II alpha 1 polypeptide pseudogene; casein kinase II alpha 1 subunit; protein kinase CK2
• UniProt Protein Name: Casein kinase II subunit alpha
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK2A1
• UniProt Synonym Gene Names: CK2A1; CK II alpha
• UniProt Entry Name: CSK21_HUMAN

NCBI Description

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

CK2A1: an ubiquitous protein kinase of the CK2 family. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. The isoforms are rarely specified in publications. Two splice variant isoforms have been found. Participates in Wnt signaling. Phosphorylates E2 ubiquitin conjugating enzyme UBC3B inducing its interaction with beta-TRCp and enhancing beta-catenin degradation. May control IkB-alpha and p27Kip1 degradation. component of a CK2-SPT16-SSRP1 complex composed of SSRP1, PT16 CK2-A1, CK2-A2 and CK2-B the complex associating following UV irradiation. Interacts with RNPS1. Mouse transgene causes mammary gland hyperplasia and lymphoma, and activation by bovine parasites leads to fatal lymphoproliferation. Expression and activity are elevated in lung tumors and breast tumors. Antisense drives apoptosis of tumor cell lines and xenografts. Involved in DNA break repair by phosphorylation of scaffold protein XRCC1, phosphorylation of BRCA1, and phosphorylation of p53 in response to UV irradiation. Drosophila CK2 (Timekeeper) is involved in circadian regulation. Phosphorylates and binds to a major component of the inclusion bodies seen in Parkinson?s patients. Inhibitors: 4,5,6,7-tetrabromobenzotriazole (TBB).

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; CK2 family

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: Sin3 complex; plasma membrane; NuRD complex; PcG protein complex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein N-terminus binding; Hsp90 protein binding; ATP binding

Biological Process: axon guidance; Wnt receptor signaling pathway; transcription, DNA-dependent; rhythmic process; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; positive regulation of cell growth; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of protein catabolic process; mitotic cell cycle; positive regulation of Wnt receptor signaling pathway