Hepatitis C Virus Combined Recombinant Protein | HCV recombinant protein
Recombinant Hepatitis C Virus Combined
Applications
ELISA, Western Blot
Purity
HCV protein is >95% pure as determined by 10% PAGE (coomassie staining).HCV protein was purified by proprietary chromatographic technique.
Synonyms
Hepatitis C Virus Combined ; Recombinant Hepatitis C Virus Combined ; HCV Combined; Hepatitis C Virus Combined Recombinant; HCV Combined ; HCV recombinant protein
Specificity
Immunoreactive with sera of HCV-infected individuals.
Purity/Purification
HCV protein is >95% pure as determined by 10% PAGE (coomassie staining).
HCV protein was purified by proprietary chromatographic technique.
HCV protein was purified by proprietary chromatographic technique.
Form/Format
50mM Na-PO4, pH-8.5, 2.4mM EDTA, 5mM DTT and 0.1% SDS.
Sequence Length
3033
Applicable Applications for HCV recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes
HCV Antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Related Product Information for HCV recombinant protein
Description: The E Coli derived 70 kDa recombinant protein contains sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E Coli as chimeric proteins with glutathione S-transferase. The most relevant proteins identified in this study were then contructed into one recombinant antigen. The protein contains the HCV nucleocapsid, NS3 genotype 1b, NS4 genotype 1b and 1a, and NS5 genotype 1b and 1a immunodominant regions.
Introduction: HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).
Introduction: HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).
Product Categories/Family for HCV recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
330,337 Da
NCBI Official Full Name
hepatitis C virus polyprotein
UniProt Protein Name
Genome polyprotein
UniProt Gene Name
HCV
UniProt Entry Name
D5MTP0_9HEPC
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Product Notes
The HCV hcv (Catalog #AAA142523) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hepatitis C Virus Combined can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). HCV Antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems. Researchers should empirically determine the suitability of the HCV hcv for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatitis C Virus Combined , Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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