Secreted Phospholipase A2-V Recombinant Protein | PLA2G5 recombinant protein

Recombinant Human Secreted Phospholipase A2-V

Cat. Num. AAA142469

• Gene Names: PLA2G5; FRFB; GV-PLA2; PLA2-10; hVPLA(2)
• Purity: Greater than 95% as determined by SDS PAGE.
• Synonyms: Secreted Phospholipase A2-V; Recombinant Human Secreted Phospholipase A2-V; Calcium-dependent phospholipase A2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase; PLA2-10; Group V phospholipase A2; GV-PLA2; MGC46205; hVPLA(2); DKFZp686C2294; sPLA2-V; PLA2G5.; PLA2G5 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS PAGE.
• Form/Format: Filtered (0.4?m) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH4.; Lyophilized (freeze-dried) powder.
• Sequence: MRGSHHHHHH GMASHMGLLD LKSMIEKVTG KNALTNYGFY GCYCGWGGRG TPKDGTDWCC WAHDHCYGRLEEKGCNIRTQ SYKYRFAWGV VTCEPGPFCH VNLCACDRKL VYCLKRNLRS YNPQYQYFPN ILCS
• Sequence Length: 138

• Reconstitution: Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Related Product Information for PLA2G5 recombinant protein

Secreted Phospholipase A2-V Human Recombinant was produced with N-terminal His-Tag. PLA2G5 His-Tagged Fusion protein is 15.5 kDa containing 118 amino acid residues of the human secreted phospholipase A2-V and 16 additional amino acid residues – His-Tag (underlined).

Product Categories/Family for PLA2G5 recombinant protein

Enzymes; Secreted Phospholipase A2

NCBI and Uniprot Product Information

• NCBI GI #: 4505853
• NCBI GeneID: 5322
• NCBI Accession #: NP_000920.1
• NCBI GenBank Nucleotide #: NM_000929.2
• UniProt Accession #: P39877; Q8N435
• NCBI Official Full Name: calcium-dependent phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group V
• NCBI Official Symbol: PLA2G5
• NCBI Official Synonym Symbols: FRFB; GV-PLA2; PLA2-10; hVPLA(2)
• NCBI Protein Information: calcium-dependent phospholipase A2; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase 5
• UniProt Protein Name: Calcium-dependent phospholipase A2
• Protein Family: Calcium-dependent phospholipase
• UniProt Gene Name: PLA2G5
• UniProt Entry Name: PA2G5_HUMAN

NCBI Description

This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Secreted, signal peptide; Secreted; Cell surface; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 1p36-p34

Cellular Component: Golgi apparatus; cell surface; perinuclear region of cytoplasm; extracellular region; plasma membrane

Molecular Function: heparin binding; calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; response to cAMP; response to cytokine stimulus; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; lipid catabolic process

Disease: Fleck Retina, Familial Benign