Standard Curve (Sample)
Human Kynurenine ELISA Kit
Kynurenine ELISA
Reactivity
Human
Synonyms
Kynurenine; Kynurenine ELISA; Kynurenine elisa kit
Reactivity
Human
Assay Type
Quantitative Competitive
Samples
Serum and EDTA-plasma samples to evaluate L-kynurenine homeostasis
Preparation and Storage
2 - 8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Kynurenine elisa kit
Background: Kynurenine is a non-proteinogenic amino acid that is produced as a metabolic intermediate during the degradation of tryptophan [1 - 5). The degradation of tryptophan is catalyzed by the inducible enzyme indolamine-2,3-dioxygenase (IDO). The product is kynurenine [4, 6 - 8). Cytokines, in particular interferon-y [5, 9, 10), influence the activity of the IDO, so that is why the kynurenine path is closely linked to the immune system [9, 11). Kynurenine can be further converted to neuroprotective kynurenic acid, but also to neurotoxiC quinolinic acid [6, 11).
Intended Uses: Enzyme immunoassay for the quantitative determination of L-kynurenine in serum and EDTA-plasma samples to evaluate L-kynurenine homeostasis. During acylation, kynurenine is activated at 37 degree C and subsequently coupled to a protein. The competitive ELISA following sample preparation is based on the microtiter plate format. The antigen (kynurenine) is bound to the solid phase. The analyte concentrations of the acylated standards, controls and samples and the analyte concentrations bound to the solid phase, compete for the available binding Sites of the antibodies. When the system is in equilibrium, the free antigens and free antigen-antibody complexes are removed by washing. The antigen-antibody complex bound to the solid phase is determined with an enzyme-labelled antibody and detected with a substrate by a colour reaction. The reaction is measured at 450 nm. The concentrations of the unknown samples are determined using a standard curve and matching the measured absorbance. Manual processing is recommended. The use of laboratory automation is the responsibility of the user. This product is not intended to clinical diagnoses.
Intended Uses: Enzyme immunoassay for the quantitative determination of L-kynurenine in serum and EDTA-plasma samples to evaluate L-kynurenine homeostasis. During acylation, kynurenine is activated at 37 degree C and subsequently coupled to a protein. The competitive ELISA following sample preparation is based on the microtiter plate format. The antigen (kynurenine) is bound to the solid phase. The analyte concentrations of the acylated standards, controls and samples and the analyte concentrations bound to the solid phase, compete for the available binding Sites of the antibodies. When the system is in equilibrium, the free antigens and free antigen-antibody complexes are removed by washing. The antigen-antibody complex bound to the solid phase is determined with an enzyme-labelled antibody and detected with a substrate by a colour reaction. The reaction is measured at 450 nm. The concentrations of the unknown samples are determined using a standard curve and matching the measured absorbance. Manual processing is recommended. The use of laboratory automation is the responsibility of the user. This product is not intended to clinical diagnoses.
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Product Notes
The Human Kynurenine (Catalog #AAA14217) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA14217 ELISA Kit recognizes Human Kynurenine. It is sometimes possible for the material contained within the vial of "Kynurenine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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