SARS Associated Coronavirus Spike Active Protein | SARS active protein
Recombinant SARS Associated Coronavirus Spike Receptor Binding Domain (306-515 a.a.), Sf9
Gene Names
S; E2
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
SARS Associated Coronavirus Spike; Recombinant SARS Associated Coronavirus Spike Receptor Binding Domain (306-515 a.a.); Sf9; SARS Spike (306-515); Coronavirus Spike Receptor Binding Domain (306-515 a.a.)Recombinant; Spike glycoprotein; S glycoprotein; Peplomer protein; E2 glycoprotein precursor; Severe acute repiratory Syndrome-related Coronavirus; SARS; SRAS-CoV; SARS-CoV1; E2; SARS active protein
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered colorless solution.
The SARS Spike (306-515) solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH7.4) and 10% Glycerol.
The SARS Spike (306-515) solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH7.4) and 10% Glycerol.
Sequence Positions
306-515aa
Sequence
ADPRVVPSGD VVRFPNITNL CPFGEVFNAT KFPSVYAWER KKISNCVADY SVLYNSTFFS TFKCYGVSAT KLNDLCFSNV YADSFVVKGD DVRQIAPGQT GVIADYNYKL PDDFMGCVLA WNTRNIDATS TGNYNYKYRY LRHGKLRPFE RDISNVPFSP DGKPCTPPAL NCYWPLNDYG FYTTTGIGYQ PYRVVVLSFE LLNAPATVCG PKLHHHHHH
Biological Activity
Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# MBS141590).
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for SARS active protein
SARS Coronavirus is an enveloped virus containing 3 outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein takes part in virus infection cycle and is the primary target of neutralizing antibodies.
SARS Spike produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 219 amino acids (306-515 aa) and having a molecular mass of 24.7kDa.SARS Spike is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
SARS Spike produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 219 amino acids (306-515 aa) and having a molecular mass of 24.7kDa.SARS Spike is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Categories/Family for SARS active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
139,125 Da
NCBI Official Full Name
Spike glycoprotein
NCBI Official Symbol
S
NCBI Official Synonym Symbols
E2
NCBI Protein Information
E2 glycoprotein precursor; spike glycoprotein
UniProt Protein Name
Spike glycoprotein
Protein Family
UniProt Gene Name
S
UniProt Synonym Gene Names
S glycoprotein
UniProt Entry Name
SPIKE_CVHSA
Uniprot Description
S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
Research Articles on SARS
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Product Notes
The SARS s (Catalog #AAA141665) is an Active Protein produced from Sf9, Baculovirus cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 306-515aa. The amino acid sequence is listed below: ADPRVVPSGD VVRFPNITNL CPFGEVFNAT KFPSVYAWER KKISNCVADY SVLYNSTFFS TFKCYGVSAT KLNDLCFSNV YADSFVVKGD DVRQIAPGQT GVIADYNYKL PDDFMGCVLA WNTRNIDATS TGNYNYKYRY LRHGKLRPFE RDISNVPFSP DGKPCTPPAL NCYWPLNDYG FYTTTGIGYQ PYRVVVLSFE LLNAPATVCG PKLHHHHHH. It is sometimes possible for the material contained within the vial of "SARS Associated Coronavirus Spike, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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