Rabbit HIV-1 gp120 Polyclonal Antibody
Polyclonal Rabbit Anti HIV-1 gp120, Purified
Applications
ELISA, Western Blot
Purity
>95% as estimated by SDS-PAGE.
Synonyms
HIV-1 gp120; Polyclonal Antibody; Polyclonal Rabbit Anti HIV-1 gp120; Purified; HIV-1 gp120 Antibody; Rabbit Antibody; HIV1gp120; HIV-I gp120; HIVIgp120; anti-HIV-1 gp120 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
>95% as estimated by SDS-PAGE.
Form/Format
Lyophilized in PBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Applicable Applications for anti-HIV-1 gp120 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Binds to recombinant HIV-1 gp120 in ELISA and Western Blot. Optimal dilutions should be determined by the customer, based on titration. Suggested starting dilutions if the protein concentration is1mg/mL are listed below: Western Blot 1:500 to 1:1000 ELISA 1:500 to 1:1000.
Solubility
The lyophilized antibody should be reconstituted in sterile dH2O. The amount of sterilized dH2O can vary depending on the final concentration desired.
Immunogen
Recombinant HIV-1 gp120 produced in baculovirus expression system.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Related Product Information for anti-HIV-1 gp120 antibody
Description: Purified Rabbit polyclonal antibody to HIV-1 gp120.
Introduction: Human immunodeficiency virus (HIV) is a retrovirus that can lead to a condition in which the immune system begins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the human immune system such as helper T cells (specifically CD4+ T cells), macrophages and dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosis in infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase so that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.
Introduction: Human immunodeficiency virus (HIV) is a retrovirus that can lead to a condition in which the immune system begins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the human immune system such as helper T cells (specifically CD4+ T cells), macrophages and dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosis in infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase so that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.
Product Categories/Family for anti-HIV-1 gp120 antibody
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Product Notes
The HIV-1 gp120 (Catalog #AAA141017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV-1 gp120 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Binds to recombinant HIV-1 gp120 in ELISA and Western Blot. Optimal dilutions should be determined by the customer, based on titration. Suggested starting dilutions if the protein concentration is1mg/mL are listed below: Western Blot 1:500 to 1:1000 ELISA 1:500 to 1:1000. Researchers should empirically determine the suitability of the HIV-1 gp120 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gp120, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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