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Mouse Thiopurine S-methyltransferase Monoclonal Antibody | anti-TPMT antibody

Mouse Anti Human Thiopurine S-methyltransferase

Applications
ELISA, Western Blot
Purity
TPMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Thiopurine S-methyltransferase; Monoclonal Antibody; Mouse Anti Human Thiopurine S-methyltransferase; TPMT Antibody; Mouse Anti Human; TPMT; EC 2.1.1.67; Thiopurine methyltransferase; anti-TPMT antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 heavy chain and k light chain
Clone Number
PAT2E7AT
Purity/Purification
TPMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sequence Length
245
Applicable Applications for anti-TPMT antibody
ELISA (EIA), Western Blot (WB)
Application Notes
TPMT antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000.
Immunogen
Anti-human TPMT mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human TPMT amino acids 1-245 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-TPMT antibody
Introduction: TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.
Product Categories/Family for anti-TPMT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,180 Da
NCBI Official Full Name
thiopurine S-methyltransferase
NCBI Official Synonym Full Names
thiopurine S-methyltransferase
NCBI Official Symbol
TPMT
NCBI Protein Information
thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase
UniProt Protein Name
Thiopurine S-methyltransferase
UniProt Gene Name
TPMT
UniProt Entry Name
TPMT_HUMAN

NCBI Description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

Uniprot Description

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.

Protein type: Methyltransferase; EC 2.1.1.67; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function: thiopurine S-methyltransferase activity

Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency

Research Articles on TPMT

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Product Notes

The TPMT tpmt (Catalog #AAA140403) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Thiopurine S-methyltransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). TPMT antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000. Researchers should empirically determine the suitability of the TPMT tpmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thiopurine S-methyltransferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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