Mouse Homeobox Protein NANOG Monoclonal Antibody | anti-NANOG antibody

Mouse Anti Human Homeobox Protein NANOG

Cat. Num. AAA140269

• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Homeobox Protein NANOG; Monoclonal Antibody; Mouse Anti Human Homeobox Protein NANOG; NANOG Antibody; Mouse Anti Human; NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; anti-NANOG antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and kappa light chain
• Clone Number: P5A10AT
• Purity/Purification: NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 305

• Applicable Applications for anti-NANOG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.
• Immunogen: Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-NANOG antibody

Introduction: NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Product Categories/Family for anti-NANOG antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 153945816
• NCBI GeneID: 79923
• NCBI Accession #: NP_079141.2
• NCBI GenBank Nucleotide #: NM_024865.3
• Molecular Weight: 32,838 Da
• NCBI Official Full Name: homeobox protein NANOG isoform 1
• NCBI Official Synonym Full Names: Nanog homeobox
• NCBI Official Symbol: NANOG
• NCBI Protein Information: homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
• UniProt Protein Name: Homeobox protein NANOG
• Protein Family: Homeobox protein
• UniProt Gene Name: NANOG
• UniProt Synonym Gene Names: hNanog
• UniProt Entry Name: NANOG_HUMAN

NCBI Description

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: DNA binding; chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: response to retinoic acid; gonad development; transcription, DNA-dependent; somatic stem cell maintenance; endodermal cell fate specification; stem cell division; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; embryonic pattern specification; mesodermal cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of cell fate commitment; cell proliferation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation