Mouse Killer Cell Immunoglobulin-Like Receptor Monoclonal Antibody | anti-KIR2DL3 antibody

Mouse Anti Human Killer Cell Immunoglobulin-Like Receptor, 2 Domains Long Cytoplasmic Tail 3

Cat. Num. AAA140180

• Gene Names: KIR2DL3; p58; NKAT; GL183; NKAT2; CD158b; NKAT2A; NKAT2B; CD158B2; KIR-K7b; KIR-K7c; KIR2DS5; KIRCL23; KIR-023GB
• Applications: ELISA, Western Blot
• Purity: KIR2DL3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Killer Cell Immunoglobulin-Like Receptor; Monoclonal Antibody; Mouse Anti Human Killer Cell Immunoglobulin-Like Receptor; 2 Domains Long Cytoplasmic Tail 3; KIR2DL3 Antibody; Killer Cell Immunoglobulin-Like Receptor 2 Domains Long Cytoplasmic Tail 3; Mouse Anti Human; Killer cell immunoglobulin-like receptor 2DL3; MHC class I NK cell receptor; Natural killer-associated transcript 2; NKAT-2; NKAT2a; NKAT2b; p58 natural killer cell receptor clone CL-6; p58 NK receptor; p58.2 MHC class-I-specific NK receptor; Killer inhibitory receptor cl 2-3; KIR-023GB; CD158 antigen-like family member B2; CD158b2 antigen; KIR2DL3; CD158B2; KIRCL23; NKAT2; p58; NKAT; GL183; CD158b; KIR-K7b; KIR-K7c; MGC129943; anti-KIR2DL3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chains and kappa light chain
• Clone Number: P190IIC311AT
• Purity/Purification: KIR2DL3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.; Sterile Filtered colorless solution.
• Sequence Length: 341

• Applicable Applications for anti-KIR2DL3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: KIR2DL3 antibody has been tested by ELISA and Western blot immunofluorescent staining with flow cytometric analysis to assure specificity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Applications include flow cytometry (5-10g/1x1000000), ELISA (1:1,000 when tested against the immunized protein) and western blot analysis (1ug/ml).
• Immunogen: Anti-human KIR2DL3 mAb, is derived from hybridization of mouse P3-x63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human KIR2DL3 amino acids 19-161 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C

Related Product Information for anti-KIR2DL3 antibody

Introduction: Killer-cell immunoglobulin-like receptors (KIRs), are a family of cell surface glycoproteins found on Natural Killer (NK) Cells, which are important cells of the immune system. They control the killing function of these cells by interacting with MHC class I molecules, which are expressed on all cell types. This interaction allows them to identify virally infected cells or tumor cells that have a distinctive low level of Class I MHC on their surface. The majority of KIRs are inhibitory, which means that their recognition of MHC suppresses the cytotoxic activity of their NK cell. Only a limited number of KIRs have the capacity to activate cells. The KIR genes are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). KIR molecules are extremely polymorphic, meaning their gene sequences differ significantly between individuals, so that different individuals have different arrays/repertoires of KIR genes. The KIR proteins are categorized by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM). Whereas KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. KIR2DL3 is an inhibitory Killer Cell Ig-like Receptor (KIR, previously called p58 KIR, cl-6, NKAT2 or KIR-K7), which recognizes class I MHC molecules (HLA-Cw1, -Cw3, -Cw7, and Cw8). KIR2DL3 inhibits the activity of NK cells thus preventing cell lysis.

Product Categories/Family for anti-KIR2DL3 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 71143139
• NCBI GeneID: 3804
• NCBI Accession #: NP_056952.2
• NCBI GenBank Nucleotide #: NM_015868.2
• Molecular Weight: 27,269 Da
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 2DL3
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
• NCBI Official Symbol: KIR2DL3
• NCBI Official Synonym Symbols: p58; NKAT; GL183; NKAT2; CD158b; NKAT2A; NKAT2B; CD158B2; KIR-K7b; KIR-K7c; KIR2DS5; KIRCL23; KIR-023GB
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL3; CD158 antigen-like family member B2; MHC class I NK cell receptor; NK-receptor; NKAT-2; killer Ig-like inhibitory receptor; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; killer inhibitory receptor cl 2-3; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; natural killer-associated transcript 2; p58 NK receptor CL-6; p58 natural killer cell receptor clone CL-6; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL3
• Protein Family: Killer cell immunoglobulin-like receptor
• UniProt Gene Name: KIR2DL3
• UniProt Synonym Gene Names: CD158B2; KIRCL23; NKAT2; NKAT-2; p58 NK receptor CL-6
• UniProt Entry Name: KI2L3_HUMAN

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

KIR2DL3: Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; receptor activity; antigen binding

Biological Process: regulation of immune response; immune response