E3 ISG15--protein ligase HERC5 (HERC5) Recombinant Protein | HERC5 recombinant protein

Recombinant Human E3 ISG15--protein ligase HERC5 (HERC5) , partial

• Gene Names: HERC5; CEB1; CEBP1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ISG15--protein ligase HERC5 (HERC5); Recombinant Human E3 ISG15--protein ligase HERC5 (HERC5); partial; HERC5 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,024

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for HERC5 recombinant protein

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.

NCBI and Uniprot Product Information

• NCBI GI #: 110825982
• NCBI GeneID: 51191
• NCBI Accession #: NP_057407.2
• NCBI GenBank Nucleotide #: NM_016323.3
• UniProt Accession #: Q9UII4; Q69G20; B2RTQ1
• Molecular Weight: 116,852 Da
• NCBI Official Full Name: E3 ISG15--protein ligase HERC5
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 5
• NCBI Official Symbol: HERC5
• NCBI Official Synonym Symbols: CEB1; CEBP1
• NCBI Protein Information: E3 ISG15--protein ligase HERC5
• UniProt Protein Name: E3 ISG15--protein ligase HERC5
• Protein Family: E3 ISG15--protein ligase
• UniProt Gene Name: HERC5
• UniProt Synonym Gene Names: CEB1; CEBP1

NCBI Description

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.