Human antiplasmin total ELISA Kit | SERPINF2 elisa kit

Human antiplasmin total antigen assay ELISA kit

Cat. Num. AAA135932

• Gene Names: SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
• Reactivity: Human
• Synonyms: antiplasmin total; Human antiplasmin total antigen assay ELISA kit; SERPINF2 elisa kit

• Reactivity: Human
• Form/Format: Complete kit
• Sequence Length: 491

• Source Info: Multiple
• Target Info: Alpha-2-antiplasmin
• Preparation and Storage: Recommended Storage: 4 degree C; Minimum Shelf Life: 12 months from manufacture, see label for expiration date; Shipping Condition: Cold pack

Related Product Information for SERPINF2 elisa kit

Alpha-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of alpha-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair. The sensitive quantitative measurement of total human alpha-2-antiplasmin antigen in samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of antiplasmin is 70 ug/ml in plasma and 47.6 ug/ml in serum. The assay measures total antiplasmin in the 0.1-100 ng/ml range. Samples giving human antiplasmin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-20,000 dilution for plasma is suggested for best results. Human antiplasmin will bind to the capture antibody coated onto a micro titer plate. Free and complexed antiplasmin will bind to the plate and will be detected by the assay. After appropriate washing steps, biotin labeled anti human antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated streptavidin. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of antiplasmin in the samples. A standard calibration curve is prepared using dilutions of purified antiplasmin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for SERPINF2 elisa kit

HUMAN ANTIPLASMIN

NCBI and Uniprot Product Information

• NCBI GI #: 115583663
• NCBI GeneID: 5345
• NCBI Accession #: NP_000925.2
• NCBI GenBank Nucleotide #: NM_000934.3
• UniProt Accession #: P08697; Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7
• Molecular Weight: 47,907 Da
• NCBI Official Full Name: alpha-2-antiplasmin isoform a
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: AAP; API; PLI; A2AP; ALPHA-2-PI
• NCBI Protein Information: alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• UniProt Protein Name: Alpha-2-antiplasmin
• Protein Family: Alpha-2-antiplasmin
• UniProt Gene Name: SERPINF2
• UniProt Synonym Gene Names: AAP; PLI; Alpha-2-AP; Alpha-2-PI
• UniProt Entry Name: A2AP_HUMAN

NCBI Description

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; platelet degranulation; negative regulation of fibrinolysis; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of cell differentiation

Disease: Alpha-2-plasmin Inhibitor Deficiency