Tryptophan dimethylallyltransferase (fgaPT2) Recombinant Protein | fgaPT2 recombinant protein

Recombinant Neosartorya fumigata Tryptophan dimethylallyltransferase (fgaPT2)

Cat. Num. AAA1357461

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Tryptophan dimethylallyltransferase (fgaPT2); Recombinant Neosartorya fumigata Tryptophan dimethylallyltransferase (fgaPT2); Tryptophan dimethylallyltransferase; EC=2.5.1.34; 4-dimethylallyltryptophan synthase; All-trans-hexaprenyl-diphosphate synthase; L-tryptophan dimethylallyl transferase; DMATS; fgaPT2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-459aa; full length protein
• Sequence: MKAANASSAE AYRVLSRAFR FDNEDQKLWW HSTAPMFAKM LETANYTTPC QYQYLITYKECVIPSLGCYP TNSAPRWLSI LTRYGTPFEL SLNCSNSIVR YTFEPINQHT GTDKDPFNTHAIWESLQHLL PLEKSIDLEW FRHFKHDLTL NSEESAFLAH NDRLVGGTIR TQNKLALDLKDGRFALKTYI YPALKAVVTG KTIHELVFGS VRRLAVREPR ILPPLNMLEE YIRSRGSKSTASPRLVSCDL TSPAKSRIKI YLLEQMVSLE AMEDLWTLGG RRRDASTLEG LSLVRELWDLIQLSPGLKSY PAPYLPLGVI PDERLPLMAN FTLHQNDPVP EPQVYFTTFG MNDMAVADALTTFFERRGWS EMARTYETTL KSYYPHADHD KLNYLHAYIS FSYRDRTPYL SVYLQSFETGDWAVANLSES KVKCQDAACQ PTSLPPDLSK TGVYYSGLH
• Sequence Length: 459

• Species: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 146323104
• NCBI GeneID: 3512716
• NCBI Accession #: XP_756141.2
• NCBI GenBank Nucleotide #: XM_751048.2
• UniProt Accession #: Q50EL0; Q4WZ62
• Molecular Weight: 52,463 Da
• NCBI Official Full Name: dimethylallyl tryptophan synthase FgaPT2
• NCBI Official Symbol: AFUA_2G18040
• NCBI Protein Information: dimethylallyl tryptophan synthase FgaPT2
• UniProt Protein Name: Tryptophan dimethylallyltransferase
• UniProt Gene Name: fgaPT2
• UniProt Synonym Gene Names: DMATS
• UniProt Entry Name: DMAW_ASPFU

Uniprot Description

Tryptophan dimethylallyltransferase; part of the gene cluster that mediates the biosynthesis of fumiclavanine C, a fungal ergot alkaloid (PubMed:15933009, PubMed:23435153, PubMed:26972831). DmaW catalyzes the first step of ergot alkaloid biosynthesis by condensing dimethylallyl diphosphate (DMAP) and tryptophan to form 4-dimethylallyl-L-tryptophan (PubMed:15870460, PubMed:23435153). The second step is catalyzed by the methyltransferase easF that methylates 4-dimethylallyl-L-tryptophan in the presence of S-adenosyl-L-methionine, resulting in the formation of 4-dimethylallyl-L-abrine (). The catalase easC and the FAD-dependent oxidoreductase easE then transform 4-dimethylallyl-L-abrine to chanoclavine-I which is further oxidized by EasD in the presence of NAD+, resulting in the formation of chanoclavine-I aldehyde (PubMed:20039019, PubMed:20526482, PubMed:21409592). EasA reduces chanoclavine-I aldehyde to dihydrochanoclavine-I aldehyde that spontaneously dehydrates to form 6,8-dimethyl-6,7-didehydroergoline (PubMed:20526482). EasG then catalyzes the reduction of 6,8-dimethyl-6,7-didehydroergoline to form festuclavine (PubMed:20526482). Hydrolysis of festuclavine by easM then leads to the formation of fumigaclavine B which is in turn acetylated by easN to fumigaclavine A (PubMed:26972831). Finally, easL catalyzes the conversion of fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus (PubMed:19672909).