Programmed cell death protein 1 (PDCD1) Active Protein | PDCD1 active protein

Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active)

Cat. Num. AAA1337527

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Programmed cell death protein 1 (PDCD1); Recombinant Human Programmed cell death protein 1 (PDCD1); partial (Active); CD279; PDCD1 active protein

• Host: Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 25-167aa; Partial
• Sequence: LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ
• Sequence Length: 170

• Activity: 1) Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 ug/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml.; 2)Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 ug/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Related Product Information for PDCD1 active protein

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Product Categories/Family for PDCD1 active protein

Cell Biology

References

A regulatory polymorphism in PDCD1 is associated with susceptibility to systemic lupus erythematosus in humans.Prokunina L., Castillejo-Lopez C., Oberg F., Gunnarsson I., Berg L., Magnusson V., Brookes A.J., Tentler D., Kristjansdottir H., Grondal G., Bolstad A.I., Svenungsson E., Lundberg I., Sturfelt G., Jonssen A., Truedsson L., Lima G., Alcocer-Varela J., Jonsson R., Gyllensten U.B., Harley J.B., Alarcon-Segovia D., Steinsson K., Alarcon-Riquelme M.E.Nat. Genet. 32:666-669(2002)
Cloning of PD-1 cDNA from activated peripheral leukocytes.He X., Xu L., Liu Y., Zeng Y.Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A.Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004)
Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.The role of the PD-1 pathway in autoimmunity and peripheral tolerance.Fife B.T., Pauken K.E.Ann. N. Y. Acad. Sci. 1217:45-59(2011)

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 20.0 kDa
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1
• UniProt Entry Name: PDCD1_HUMAN

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: external side of plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; signal transducer activity

Biological Process: apoptosis; humoral immune response; multicellular organismal development; negative regulation of apoptosis; negative regulation of tolerance induction; signal transduction; T cell costimulation

Disease: Systemic Lupus Erythematosus