Centromere protein S (APITD1) Recombinant Protein | APITD1 recombinant protein

Recombinant Human Centromere protein S (APITD1)

Cat. Num. AAA1315216

• Gene Names: CENPS-CORT; APITD1-CORT
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Centromere protein S (APITD1); Recombinant Human Centromere protein S (APITD1); APITD1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-138, Full length protein
• Sequence: MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCENFAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSEDGSKNSRQPAEAGVVESEN
• Sequence Length: 138

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for APITD1 recombinant protein

This gene was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. Alternative splicing of this gene results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 395394019
• NCBI GeneID: 100526739
• NCBI Accession #: NP_001257446.1
• NCBI GenBank Nucleotide #: NM_001270517.1
• UniProt Accession #: Q8N2Z9; Q8NFE5; Q8NFG5
• Molecular Weight: 9,074 Da
• NCBI Official Full Name: APITD1-CORT protein isoform 4
• NCBI Official Synonym Full Names: CENPS-CORT readthrough
• NCBI Official Symbol: CENPS-CORT
• NCBI Official Synonym Symbols: APITD1-CORT
• NCBI Protein Information: APITD1-CORT protein
• UniProt Protein Name: Centromere protein S
• Protein Family: Centromere protein
• UniProt Gene Name: CENPS
• UniProt Synonym Gene Names: CENP-S

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring APITD1 (apoptosis-inducing, TAF9-like domain 1) and CORT (cortistatin) genes. Alternative splicing results in multiple transcript variants, two of which encode fusion proteins that share sequence identity with the products of each individual gene. [provided by RefSeq, Aug 2011]

Uniprot Description

DNA-binding component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PubMed:20347428, PubMed:20347429). In complex with CENPX (MHF heterodimer), crucial cofactor for FANCM in both binding and ATP-dependent remodeling of DNA. Stabilizes FANCM (PubMed:20347428, PubMed:20347429). In complex with CENPX and FANCM (but not other FANC proteins), rapidly recruited to blocked forks and promotes gene conversion at blocked replication forks (PubMed:20347428). In complex with CENPT, CENPW and CENPX (CENP-T-W-S-X heterotetramer), involved in the formation of a functional kinetochore outer plate, which is essential for kinetochore-microtubule attachment and faithful mitotic progression (PubMed:19620631). As a component of MHF and CENP-T-W-S-X complexes, binds DNA and bends it to form a nucleosome-like structure (PubMed:20347428, PubMed:22304917). DNA-binding function is fulfilled in the presence of CENPX, with the following preference for DNA substates: Holliday junction > double-stranded > splay arm > single-stranded. Does not bind DNA on its own (PubMed:20347428, PubMed:20347429).