DNA excision repair protein ERCC-8 (ERCC8) Recombinant Protein | ERCC8 recombinant protein

Recombinant Human DNA excision repair protein ERCC-8 (ERCC8)

Cat. Num. AAA1313369

• Gene Names: ERCC8; CSA; CKN1; UVSS2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA excision repair protein ERCC-8 (ERCC8); Recombinant Human DNA excision repair protein ERCC-8 (ERCC8); ERCC8 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-396, Full length protein
• Sequence: MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGGGINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRRASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSNGENTLVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHYKTVDCCVFQSNFQELYSGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSSDEEG
• Sequence Length: 396

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for ERCC8 recombinant protein

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

NCBI and Uniprot Product Information

• NCBI GI #: 4557467
• NCBI GeneID: 1161
• NCBI Accession #: NP_000073.1
• NCBI GenBank Nucleotide #: NM_000082.3
• UniProt Accession #: Q13216; Q6FHX5; Q96GB9; B2RB64
• Molecular Weight: 23,182 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-8 isoform 1
• NCBI Official Synonym Full Names: ERCC excision repair 8, CSA ubiquitin ligase complex subunit
• NCBI Official Symbol: ERCC8
• NCBI Official Synonym Symbols: CSA; CKN1; UVSS2
• NCBI Protein Information: DNA excision repair protein ERCC-8
• UniProt Protein Name: DNA excision repair protein ERCC-8
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC8
• UniProt Synonym Gene Names: CKN1; CSA

NCBI Description

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes.