Western Blot (WB)
(Western blot analysis of extracts from HL60 cells, using phospho-MAP2K6-S207 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit anti-Human, Rat MAP2K6-S207 Antibody | anti-MAP2K6 antibody
Phospho-MAP2K6-S207 Polyclonal Antibody
IHC: 1:50 - 1:100
IF: 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts from HL60 cells, using phospho-MAP2K6-S207 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts from HL60 cells, using phospho-MAP2K6-S207 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.
Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; STE group; STE7 family
Chromosomal Location of Human Ortholog: 17q24.3
Cellular Component: nucleoplasm; cytoskeleton; cytosol
Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: peptidyl-tyrosine phosphorylation; DNA damage induced protein phosphorylation; positive regulation of apoptosis; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; ovulation cycle process; regulation of transcription, DNA-dependent; cell cycle arrest; toll-like receptor 4 signaling pathway; cardiac muscle contraction; response to drug; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; positive regulation of prostaglandin secretion; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; toll-like receptor 9 signaling pathway; positive regulation of nitric-oxide synthase biosynthetic process
Research Articles on MAP2K6
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Product Notes
The MAP2K6 map2k6 (Catalog #AAA128549) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MAP2K6-S207 Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2K6-S207 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:1000 IHC: 1:50 - 1:100 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the MAP2K6 map2k6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2K6-S207, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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